Comparison of three vascular endothelial markers in the evaluation of microvessel density in breast cancer.
Comparison of three vascular endothelial markers in the evaluation of microvessel density in breast cancer.
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发表时间：
2009
影响因子：
0.4
通讯作者：
B. B. da Silva-B.;P. V. Lopes-Costa;A. R. Dos Santos;E. C. de Sousa-Júnior;A. Alencar;C. G. Pires;M. Rosal
中科院分区：
文献类型：
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作者：
B. B. da Silva-B.;P. V. Lopes-Costa;A. R. Dos Santos;E. C. de Sousa-Júnior;A. Alencar;C. G. Pires;M. Rosal
目的比较抗凝血因子VIII相关抗原、抗CD 31和抗CD 34单克隆抗体检测乳腺癌组织中微血管密度的差异。方法：对23例确诊为II期乳腺癌的绝经后妇女进行了确定性手术治疗。使用的单克隆抗体是抗因子VIII、抗CD 31和抗CD 34。在10个随机视野（200 x）中对微血管密度最高的区域进行微血管计数。使用Kruskal-Wallis非参数检验（p < 0.05）分析数据。结果抗凝血因子VIII、抗CD 31和抗CD 34的平均微血管密度分别为4.16 +/- 0.38、4.09 +/- 0.23和6.59 +/- 0.42。通过抗-CD 34评估的微血管密度显著大于通过抗-CD 31或抗-因子VIII检测的微血管密度（p < 0.0001）。抗CD 31和抗因子VIII之间无统计学显著差异（p = 0.4889）。结论与抗CD 31和抗凝血因子VIII相关抗原相比，抗CD 34染色的微血管密度更大，染色更强烈。
PURPOSE To evaluate the microvessel density by comparing the performance of anti-factor VIII-related antigen, anti-CD31 and anti-CD34 monoclonal antibodies in breast cancer. METHODS Twenty-three postmenopausal women diagnosed with Stage II breast cancer submitted to definitive surgical treatment were evaluated. The monoclonal antibodies used were anti-factor VIII, anti-CD31 and anti-CD34. Microvessels were counted in the areas of highest microvessel density in ten random fields (200 x). The data were analyzed using the Kruskal-Wallis nonparametric test (p < 0.05). RESULTS Mean microvessel densities with anti-factor VIII, anti-CD31 and anti-CD34 were 4.16 +/- 0.38, 4.09 +/- 0.23 and 6.59 +/- 0.42, respectively. Microvessel density as assessed by anti-CD34 was significantly greater than that detected by anti-CD31 or anti-factor VIII (p < 0.0001). There was no statistically significant difference between anti-CD31 and anti-factor VIII (p = 0.4889). CONCLUSION The density of stained microvessels was greater and staining was more intense with anti-CD34 compared to anti-CD31 and anti-factor VIII-related antigen.