Scenedesmus sp NJ-1 isolated from Antarctica: a suitable renewable lipid source for biodiesel production
Scenedesmus sp NJ-1 isolated from Antarctica: a suitable renewable lipid source for biodiesel production
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DOI：
10.1007/s11274-012-1132-0
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发表时间：
2012-11-01
影响因子：
4.1
通讯作者：
Hu, Hanhua
中科院分区：
文献类型：
--
作者：
Chen, Zhuo;Gong, Yangmin;Hu, Hanhua
微藻脂是生物柴油生产中很有前途的替代原料。从南极分离的富油淡水微藻Scenedesmus sp. NJ-1被确定为生产生物柴油的合适人选。该菌株可在4 ~ 35℃的温度范围内生长，培养基中硝酸盐浓度有规律地降低，通过薄层色谱和BODIPY 505/515荧光染色检测到批量培养条件下积累了大量的甘油三酯。Scenedesmus sp. NJ-1的平均生物量生产力为0.105 g l(-1) d(-1)（干重），在第28天达到了几乎最高的脂质含量（占干重的35%）。中性脂肪占总脂肪的78%，C18:1 （n-9）和C16:0是总脂肪中的主要脂肪酸，分别占生长36 d时总脂肪酸的37%和20%。这些结果表明，Scenedesmus sp. NJ-1是生产生物柴油的良好微藻油来源。
Microalgal lipids are promising alternative feedstocks for biodiesel production. Scenedesmus sp. NJ-1, an oil-rich freshwater microalga isolated from Antarctica, was identified to be a suitable candidate to produce biodiesel in this study. This strain could grow at temperatures ranging from 4 to 35 A degrees C. With regular decrease in nitrate concentration in the medium, large quantities of triacylglycerols accumulated under batch culture conditions detected by thin layer chromatography and BODIPY 505/515 fluorescent staining. Scenedesmus sp. NJ-1 achieved the average biomass productivity of 0.105 g l(-1) d(-1) (dry weight) and nearly the highest lipid content (35 % of dry cell weight) was reached at day 28 in the batch culture. Neutral lipids accounted for 78 % of total lipids, and C18:1 (n-9), C16:0 were the major fatty acids in total lipids, composing 37 and 20 % of total fatty acids of Scenedesmus sp. NJ-1 grown for 36 days, respectively. These results suggested that Scenedesmus sp. NJ-1 was a good source of microalgal oils for biodiesel production.