Identification of selenium species in selenium-enriched Lens esculenta plants by using two-dimensional liquid chromatography-inductively coupled plasma mass spectrometry and [77Se]selenomethionine selenium oxide spikes
Identification of selenium species in selenium-enriched Lens esculenta plants by using two-dimensional liquid chromatography-inductively coupled plasma mass spectrometry and [77Se]selenomethionine selenium oxide spikes
复制标题
DOI：
10.1016/j.chroma.2006.11.031
复制
发表时间：
2007-01-19
影响因子：
4.1
通讯作者：
Camara, Carmen
中科院分区：
文献类型：
--
作者：
Pedrero, Zoyne;Encinar, Jorge Ruiz;Camara, Carmen
研究了含无机硒Na2SeO3和NaSeO4水培培养富硒水藻中硒的形态形成。生长16天后，收集植株并将其分成根和茎两部分进行分析，以鉴定和量化硒的种类。采用阴离子交换柱（PRP-X100）和尺寸排除/离子交换柱（Shodex Asahipak）耦合电感耦合等离子体质谱（ICP-MS）对酶提取物进行物种形成研究。需要使用两个独立的色谱机制明确的物种鉴定证明。此外，使用[Se-77]硒代蛋氨酸氧化硒尖峰被证明是区分硒代蛋氨酸氧化硒和硒代半胱氨酸的关键。(c) 2006 Elsevier b.v.版权所有
Selenium speciation in Se-enriched Lens esculenta grown in hydroponic culture containing inorganic selenium as Na2SeO3 and NaSeO4 was performed. After 16 days of growth, the plants were collected and divided in two parts, roots and stems and then analysed to identify and quantify selenium species. Speciation studies of the enzymatic extracts were carried out by using anion-exchange (PRP-X100) and size-exclusion/ionexchange (Shodex Asahipak) columns coupled to inductively coupled plasma mass spectrometry (ICP-MS). The need of using two independent chromatographic mechanisms for unambiguous species identification is demonstrated. Moreover, the use of a [Se-77]selenomethionine selenium oxide spike turned out to be critical to discriminate between selenium selenomethioine selenium oxide and selenocysteine. (c) 2006 Elsevier B.V All rights reserved.