Three mating type-like loci in Candida glabrata.
Three mating type-like loci in Candida glabrata.
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光滑念珠菌中的三个交配型样基因座。
DOI：
10.1128/ec.2.2.328-340.2003
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
Soll,DavidR
中科院分区：
文献类型：
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作者：
Srikantha,Thyagarajan;Lachke,SalilA;Soll,DavidR
光滑念珠菌（Candida glabrata）是人类第二大常见的念珠菌属，具有3个交配型样（mating type-like，MTL）基因座（MTL 1、MTL 2和MTL 3）。这些基因座含有成对的MTL基因，其各自的编码区位于互补的Crick和沃森DNA链上。每对基因由一个共享的基因间启动子区分开，与酿酒酵母交配型基因座的结构相同。其中MTL 1和MTL 2基因座在不同菌株中具有MTL α 1/MTL α 2构型或MTL α1/MTLα2构型。38名受试者中除1名外均为C。光滑菌株为aaα或aαα。一个测试菌株是α。根据交配型基因型、MTL 1或MTL 2位点的MTL基因以及携带MTL 1或MTL 2的XbaI片段的大小，将C. glabratastries（I，II，III和IV）进行了区分。北方分析显示，菌株要么是a-表达子，要么是α-表达子，并且表达总是反映MTL 1或MTL 2基因座的基因型，具体取决于类别。因此，在每一类中的表达模式与在酿酒酵母中观察到的相似，酿酒酵母具有两个沉默盒基因座HMR和HML以及表达基因座MAT。在α-表达菌株中，核心表型之间的高频表型转换调节了MTL表达水平，这表明核心表型转换与交配之间可能存在关系。
Candida glabrata, the second most prevalentCandidaspecies colonizing humans, possesses three mating type-like (MTL)loci (MTL1,MTL2, andMTL3). These loci contain pairs ofMTLgenes with their respective coding regions on complementary Crick and Watson DNA strands. Each pair of genes is separated by a shared intergenic promoter region, the same configuration found at the mating type loci ofSaccharomyces cerevisiae. Two of theMTLloci,MTL1andMTL2, contain either theMTLa1/MTLa2 configuration or theMTLα1/MTLα2 configuration in different strains. All but one of the 38 testedC. glabratastrains were either aaα or aαα. One test strain was ααα. Based on the mating type genotype, theMTLgenes at theMTL1orMTL2loci, and the size of theXbaI fragment harboringMTL1orMTL2, four classes ofC. glabratastrains (I, II, III, and IV) were distinguished. Northern analysis revealed that strains were either a-expressors or α-expressors and that expression always reflected the genotype of either theMTL1orMTL2locus, depending on the class. The expression pattern in each class, therefore, is similar to that observed inS. cerevisiae, which harbors two silent cassette loci,HMRandHML, and the expression locusMAT. High-frequency phenotypic switching between core phenotypes in an α-expressing, but not in an a-expressing, strain modulated the level ofMTLexpression, suggesting a possible relationship between core phenotypic switching and mating.