Nanoporous Anodic Alumina-Based Sensor for miR-99a-5p Detection as an Effective Early Breast Cancer Diagnostic Tool
Nanoporous Anodic Alumina-Based Sensor for miR-99a-5p Detection as an Effective Early Breast Cancer Diagnostic Tool
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DOI：
10.1021/acssensors.0c02222
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发表时间：
2021-02-18
期刊：
影响因子：
8.9
通讯作者：
Martinez-Manez, Ramon
中科院分区：
文献类型：
--
作者：
Garrido-Cano, Iris;Pla, Luis;Martinez-Manez, Ramon
循环中的microRNAs已成为潜在的诊断生物标志物。此前，microRNA miR-99a-5p的失控已被描述为早期乳腺癌的有效生物标志物。在这里，我们提出了一种新型的基于纳米孔阳极氧化铝(NAA)的生物传感器，可以高灵敏度和选择性地检测等离子体miR-99a-5P。NAA孔中负载有罗丹明B，并被特定的寡核苷酸覆盖，该寡核苷酸能够阻止货物释放，直到靶子存在。在miR-99a-5p存在的情况下，封顶寡核苷酸识别miR-99a-5p序列并将其置换，从而释放包裹的染料。这种方法能够成功地区分健康对照和乳腺癌患者，即使是在早期阶段也是如此，这表明该系统是一种很有前途的乳腺癌检测工具。
Circulating microRNAs have emerged as potential diagnostic biomarkers. The deregulation of the microRNA miR-99a-5p has been previously described as an effective biomarker of early breast cancer. Herein, we present a new nanoporous anodic alumina (NAA)-based biosensor that can detect plasma miR-99a-5p with high sensitivity and selectivity. NAA pores are loaded with rhodamine B and capped with a specific oligonucleotide that is able to block cargo release until the target is present. In the presence of miR-99a-5p, the capping oligonucleotide recognizes the miR-99a-5p sequence and displaces it allowing the release of the encapsulated dye. This method is able to successfully distinguish healthy controls from breast cancer patients, even at early stages with high efficiency, showing the presented system as a promising tool for breast cancer detection.