Ultrastructure of last larval instar fat body cells of Pachycondyla (= Neoponera) villosa (Formicidae: Ponerinae): cytochemical and chemical analysis
Ultrastructure of last larval instar fat body cells of Pachycondyla (= Neoponera) villosa (Formicidae: Ponerinae): cytochemical and chemical analysis
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Pachycondyla (= Neoponera) villosa (Formicidae: Ponerinae) 最后幼虫龄脂肪体细胞的超微结构：细胞化学和化学分析
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
K. Jaffe
中科院分区：
文献类型：
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作者：
F. Zara;F. Caetano;A. Cabrera;K. Jaffe
本文报道了厚髁棘（D Neoponera）最后幼虫脂肪体细胞（滋养细胞）的超微结构。细胞质局限于细胞周围、液泡、蛋白颗粒、脂滴之间和细胞核周围的小条。在细胞化学上，用乙醇磷钨酸技术（EPTA）观察了蛋白质颗粒和细胞核中碱性氨基酸的存在。脂滴染色为不饱和脂。气相色谱和质谱进一步证实了这一结果，其中不饱和脂肪酸被确定为油酸和亚油酸，饱和脂肪酸如棕榈酸和硬脂酸。在脂肪体中也检测到碳水化合物（糖原）。糖原以颗粒形式存在于脂滴之间，有时与脂滴相连。
The ultrastructure of the fat body cells (trophocytes) of the last larval instar of Pachy- condyla (D Neoponera) villosa is presented. The cytoplasm is restricted to the cell periphery and to the smaller strips among the vacuoles, protein granules, lipid droplets, and around the nucleus. Cyto- chemically, the presence of basic amino acids in the protein granules and in the nuclei was observed by using the ethanolic phosphotungstic acid technique (EPTA). The lipid droplets stained for unsatu- rated lipids. This result was further cone rmed by gas chromatography and mass spectrometry, where the unsaturated fatty acids were identie ed as oleic and linoleic acids together with saturated fatty acids such as palmitic and stearic acid. Carbohydrates (glycogen) were also detected in the fat body. The glycogen is present asparticles distributed among the lipid droplets and sometimes attached to them.