A FLORAL TRANSMITTING TISSUE-SPECIFIC GLYCOPROTEIN ATTRACTS POLLEN TUBES AND STIMULATES THEIR GROWTH
A FLORAL TRANSMITTING TISSUE-SPECIFIC GLYCOPROTEIN ATTRACTS POLLEN TUBES AND STIMULATES THEIR GROWTH
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DOI：
10.1016/0092-8674(95)90427-1
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发表时间：
1995-08-11
期刊：
影响因子：
64.5
通讯作者：
WU, HM
中科院分区：
文献类型：
--
作者：
CHEUNG, AY;WANG, H;WU, HM
花粉管在雌蕊组织的细胞外基质中定向伸长，将雄配子从顶端的柱头运送到基部的子房进行受精。花粉管在雌蕊中定向生长的分子机制还不清楚。我们从烟草花柱传递组织中纯化了一种糖蛋白TTS，它支持柱头和子房之间的花粉管生长。TTS蛋白属于阿拉伯半乳聚糖蛋白家族，并且它们在体外容易以头对尾的方式折叠成寡聚体形式。TTS蛋白在体外刺激花粉管生长并吸引在半体内培养系统中生长的花粉管。在体内，在转基因植物中，由于反义抑制或正义共抑制，TTS蛋白水平显著降低，花粉管生长速率降低。这些结果确定TTS蛋白作为雌蕊组分，积极有助于花粉管生长。
Pollen tubes elongate directionally in the extracellular matrix of pistil tissues to transport the male gametes from the apically located stigma to the basally located ovary for fertilization. The molecular mechanisms underlying directional pollen tube growth in the pistil are poorly understood. We have purified a glycoprotein, TTS, from tobacco stylar transmitting tissue, which supports pollen tube growth between the stigma and the ovary. TTS proteins belong to the arabinogalactan protein family, and they polymerize readily in vitro in a head-to-tail fashion into oligomeric forms. TTS proteins stimulate pollen tube growth in vitro and attract pollen tubes grown in a semi-in vivo culture system. In vivo, the pollen tube growth rate is reduced in transgenic plants that have significantly reduced levels of TTS proteins as a result of either antisense suppression or sense cosuppression. These results identify TTS protein as a pistil component that positively contributes to pollen tube growth.