Effect of acute swim stress on plasma corticosterone and brain monoamine levels in bidirectionally selected DxH recombinant inbred mouse strains differing in fear recall and extinction
Effect of acute swim stress on plasma corticosterone and brain monoamine levels in bidirectionally selected DxH recombinant inbred mouse strains differing in fear recall and extinction
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DOI：
10.3109/10253890.2014.954104
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发表时间：
2014-12-01
影响因子：
2.3
通讯作者：
Yilmazer-Hanke, Deniz
中科院分区：
文献类型：
--
作者：
Browne, Caroline A.;Hanke, Joachim;Yilmazer-Hanke, Deniz
在恐惧相关行为不同的小鼠品系中检查了压力引起的血浆皮质酮和中枢单胺水平的变化。使用了两种具有 DBA/2J 背景的 DxH 重组近交系小鼠品系，它们最初是为了高 (H-FSS) 和低恐惧敏感声惊跳反射 (L-FSS) 而培育的。研究人员研究了杏仁核、海马、内侧前额皮质、纹状体、下丘脑和大脑中去甲肾上腺素、多巴胺和血清素及其代谢物 3,4-二羟基苯乙酸 (DOPAC)、高香草酸 (HVA) 和 5-羟基吲哚乙酸 (5-HIAA) 的水平。 脑干。 H-FSS 小鼠在听觉恐惧调节测试中表现出恐惧水平增加和恐惧消退（会话内）缺陷，在急性强迫游泳压力测试中表现出抑郁样行为。在基础条件下，他们的组织去甲肾上腺素和血清素水平较高，而多巴胺和血清素周转率较低，尽管他们对压力引起的神经递质代谢变化基本上不敏感。相比之下，急性游泳压力增加了单胺水平，但降低了不太害怕的 L-FSS 小鼠的周转率。与 H-FSS 小鼠相比，L-FSS 小鼠还表现出基础和应激诱导的皮质酮水平较高的趋势，以及应激后 30 分钟下丘脑和脑干中去甲肾上腺素和血清素的增加。此外，急性应激时，两种品系的内侧前额皮质和纹状体的多巴胺能系统被不同程度地激活。因此，H-FSS 小鼠表现出与恐惧表型或慢性应激状态相一致的基础去甲肾上腺素组织水平增加。 H-FSS 小鼠皮质酮水平低和单胺对压力的反应较差可能表明其机制与主要恐惧症或创伤后应激障碍中发现的机制相似。
Stress-induced changes in plasma corticosterone and central monoamine levels were examined in mouse strains that differ in fear-related behaviors. Two DxH recombinant inbred mouse strains with a DBA/2J background, which were originally bred for a high (H-FSS) and low fear-sensitized acoustic startle reflex (L-FSS), were used. Levels of noradrenaline, dopamine, and serotonin and their metabolites 3,4-dihydroxyphenyacetic acid (DOPAC), homovanillic acid (HVA), and 5-hydroxyindoleacetic acid (5-HIAA) were studied in the amygdala, hippocampus, medial prefrontal cortex, striatum, hypothalamus and brainstem. H-FSS mice exhibited increased fear levels and a deficit in fear extinction (within-session) in the auditory fear-conditioning test, and depressive-like behavior in the acute forced swim stress test. They had higher tissue noradrenaline and serotonin levels and lower dopamine and serotonin turnover under basal conditions, although they were largely insensitive to stress-induced changes in neurotransmitter metabolism. In contrast, acute swim stress increased monoamine levels but decreased turnover in the less fearful L-FSS mice. L-FSS mice also showed a trend toward higher basal and stress-induced corticosterone levels and an increase in noradrenaline and serotonin in the hypothalamus and brainstem 30 min after stress compared to H-FSS mice. Moreover, the dopaminergic system was activated differentially in the medial prefrontal cortex and striatum of the two strains by acute stress. Thus, H-FSS mice showed increased basal noradrenaline tissue levels compatible with a fear phenotype or chronic stressed condition. Low corticosterone levels and the poor monoamine response to stress in H-FSS mice may point to mechanisms similar to those found in principal fear disorders or post-traumatic stress disorder.