Expression of stress-response proteins upon whitefly-mediated inoculation of Tomato yellow leaf curl virus in susceptible and resistant tomato plants
Expression of stress-response proteins upon whitefly-mediated inoculation of Tomato yellow leaf curl virus in susceptible and resistant tomato plants
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DOI：
10.1094/mpmi-20-11-1376
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发表时间：
2007-11-01
影响因子：
3.5
通讯作者：
Czosnek, Henryk
中科院分区：
文献类型：
--
作者：
Gorovits, Rena;Akad, Fouad;Czosnek, Henryk
为了更好地理解番茄对烟粉虱（Bemisia tabaci，B生物型）传播的番茄黄化曲叶病毒（TYLCV）的抗性的本质，将烟粉虱和TYLCV视为生物胁迫的特例，而将病毒抗性视为成功应对这些胁迫的特例。两个自交系的番茄从同一育种计划，使用茄habrochaites作为TYLCV抗性来源，一个敏感和其他抗性，被用来比较的关键蛋白质的表达参与在不同阶段的植物响应与应力：丝裂原活化蛋白激酶（MAPKs），细胞热休克蛋白（HSPs，蛋白酶），和病程相关（PR）蛋白。两种生物胁迫-非含病毒粉虱喂养和病毒感染含病毒昆虫-导致MAPKs，HSP和叶绿体蛋白酶FtsH（但不是叶绿体蛋白酶ClpC）的丰度缓慢下降，并诱导PR蛋白，β-1，3-葡聚糖酶和过氧化物酶的活性。这种下降是不明显的病毒抗性比病毒敏感线。相反，粉虱侵染和病毒感染，接种真菌核盘菌诱导的应激蛋白的研究，随后下降的快速积累;病毒敏感和抗番茄品系的表现相似，在响应真菌。
To better understand the nature of resistance of tomato to the whitefly (Bemisia tabaci, B biotype)-transmitted Tomato yellow leaf curl virus (TYLCV), whiteflies and TYLCV were considered as particular cases of biotic stresses and virus resistance as a particular case of successful response to these stresses. Two inbred tomato lines issued from the same breeding program that used Solanum habrochaites as a TYLCV resistance source, one susceptible and the other resistant, were used to compare the expression of key proteins involved at different stages of the plant response with stresses: mitogen-activated protein kinases (MAPKs), cellular heat shock proteins (HSPs, proteases), and pathogenesis-related (PR) proteins. The two biotic stresses-non-viruliferous whitefly feeding and virus infection with viruliferous insects-led to a slow decline in abundance of MAPKs, HSPs, and chloroplast protease FtsH (but not chloroplast protease ClpC), and induced the activities of the PR proteins, beta-1,3-glucanase, and peroxidase. This decline was less pronounced in virus-resistant than in virus-susceptible lines. Contrary to whitefly infestation and virus infection, inoculation with the fungus Sclerotinia sclerotiorum induced a rapid accumulation of the stress proteins studied, followed by a decline; the virus-susceptible and -resistant tomato lines behaved similarly in response to the fungus.