On-line simultaneous removal of human serum albumin and enrichment of doxazosin using a weak cation-exchange monolithic column.
On-line simultaneous removal of human serum albumin and enrichment of doxazosin using a weak cation-exchange monolithic column.
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DOI：
10.1016/j.chroma.2006.07.029
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发表时间：
2006-10
期刊：
影响因子：
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通讯作者：
Gengliang Yang;Haiyan Liu;Yihua Zhang;Sumin Wang;Junfa Yin;Boying Yin;Yi Chen
中科院分区：
文献类型：
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作者：
Gengliang Yang;Haiyan Liu;Yihua Zhang;Sumin Wang;Junfa Yin;Boying Yin;Yi Chen
用乙二胺和一氯乙酸对甲基丙烯酸缩水甘油酯-乙二醇二甲基丙烯酸酯（GMA-EDMA）整体柱进行改性，制备了弱阳离子交换整体柱。考察了该色谱柱的性能，该色谱柱具有低反压、快速分析的特点。利用该整体柱对人血清白蛋白（HSA）溶液和血浆样品中的多沙唑嗪进行了在线检测，得到了整体柱的萃取效率和最大上样量。结果表明，该整体柱可同时实现对人血清白蛋白溶液和人血浆中微量药物的脱蛋白和富集，为血液中药物的分析提供了一种简单、廉价、有效、环境友好的方法。
A weak cation-exchange monolithic column was prepared by modifying the GMA-EDMA (glycidyl methacrylate-co-ethylene glycol dimethacrylate) monoliths with ethylenediamine and monochloracetic acid. The properties of the column were investigated; the column exhibited the ability of low backpressure and fast analysis. Using this monolithic column, trace doxazosin in human serum albumin (HSA) solution and plasma samples has been on-line tested, the extraction efficiency and the maximum loading capacity of the monolithic column were obtained. The results showed that the monolithic column could realize deproteinization and trace drug enrichment simultaneously in the HSA solution and human plasma, which provided a simple, cheap, effective and friendly to environment method for assaying drugs in the blood.