Tobacco mosaic virus infection disproportionately impacts phloem associated translatomes in Arabidopsis thaliana and Nicotiana benthamiana
Tobacco mosaic virus infection disproportionately impacts phloem associated translatomes in Arabidopsis thaliana and Nicotiana benthamiana
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DOI：
10.1016/j.virol.2017.07.002
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发表时间：
2017-10-01
期刊：
影响因子：
3.7
通讯作者：
Culver, James N.
中科院分区：
文献类型：
--
作者：
Collum, Tamara D.;Culver, James N.
在这项研究中，我们使用维管特异启动子和翻译核糖体亲和纯化策略来鉴定烟草花叶病毒(TMV)侵染系统寄主拟南芥(Arabiopsis Thaliana)、山达拉(Shandara)和本氏烟草(Nictiana Benthamiana)的韧皮部相关翻译组。结果表明，在这两种寄主中，韧皮部特异的翻译体比非韧皮部的翻译体中发生的翻译体基因改变的数量至少高出四倍。这一发现表明韧皮部是烟草花叶病毒侵染的关键反应组织。此外，寄主对翻译组改变的比较揭示了韧皮部对感染反应的相似性和差异性，既包括与促进感染和病毒传播有关的保守的病毒诱导的韧皮部改变，也包括反映症状反应差异的寄主特异性改变。综合这些结果，表明韧皮部组织在调节和控制宿主对病毒感染的反应中发挥了不成比例的作用。
In this study we use vascular specific promoters and a translating ribosome affinity purification strategy to identify phloem associated translatome responses to infection by tobacco mosaic virus (TMV) in systemic hosts Arabidopsis thaliana ecotype Shandara and Nicotiana benthamiana. Results demonstrate that in both hosts the number of translatome gene alterations that occurred in response to infection is at least four fold higher in phloem specific translatomes than in non-phloem translatomes. This finding indicates that phloem functions as a key responsive tissue to TMV infection. In addition, host comparisons of translatome alterations reveal both similarities and differences in phloem responses to infection, representing both conserved virus induced phloem alterations involved in promoting infection and virus spread as well as host specific alterations that reflect differences in symptom responses. Combined these results suggest phloem tissues play a disproportion role in the mediation and control of host responses to virus infection.