Parathyroid hormone (PTH)/PTH-related peptide receptor messenger ribonucleic acids are widely distributed in rat tissues.
Parathyroid hormone (PTH)/PTH-related peptide receptor messenger ribonucleic acids are widely distributed in rat tissues.
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DOI：
10.1210/endo.133.2.8393771
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发表时间：
1993-08
期刊：
影响因子：
4.8
通讯作者：
Pablo Ureña;Xiang-Fu Kong;A. Abou-Samra;H. Jüppner;H. Kronenberg;J. T. Potts;G. Segre
中科院分区：
文献类型：
--
作者：
Pablo Ureña;Xiang-Fu Kong;A. Abou-Samra;H. Jüppner;H. Kronenberg;J. T. Potts;G. Segre
PTH/PTH-related peptide（PTHrP）受体mRNA广泛分布于大鼠组织中。PTH和PTHrP，一种负责与癌症相关的高钙血症的肽，等同地结合最初从大鼠骨和负鼠肾细胞cDNA文库中克隆的共同受体。本研究以大鼠PTH/PTHrP受体cDNA为探针，采用北方杂交技术，对大鼠不同组织中PTH/PTHrP受体的表达进行了研究。PTH/PTHrP受体转录物在PTH靶组织、肾和骨中高度表达。然而，受体转录物也在许多其他组织中表达，包括主动脉、肾上腺、膀胱、脑、小脑、乳腺、心脏、回肠、肝、肺、骨骼肌、卵巢、胎盘、皮肤、脾、胃、子宫和睾丸。大多数组织中的主要转录物大小为2.3-2.5个酶。在肾脏和肝脏中观察到至少两种较大的mRNA，并且在肾脏、皮肤和睾丸中发现较小的转录物。最丰富的睾丸转录物大小为1.4-1.5个酶，它与两种不同的cDNA探针杂交，所述cDNA探针编码从推定的第四跨膜结构域到其C末端的受体序列的部分。然而，它不与编码受体序列的前107个残基的探针杂交。尽管PTH/PTHrP受体mRNA在肾脏和骨骼中高度表达，但其广泛的组织分布表明PTH和/或PTHrP具有其他生理作用，特别是在这些其他组织中。导致不同大小的PTH/PTHrP受体转录本的组织特异性表达的机制以及这些mRNA的功能仍有待确定。
PTH/PTH-related peptide (PTHrP) receptor mRNAs are widely distributed in rat tissues. PTH and PTHrP, a peptide responsible for hypercalcemia associated with cancers, bind equivalently to common receptors that initially were cloned from rat bone and opossum renal cell cDNA libraries. In this study we used rat PTH/PTHrP receptor cDNA to probe for receptor expression in different rat tissues by Northern blot analysis. PTH/PTHrP receptor transcripts are highly expressed in PTH target tissues, kidney and bone. Receptor transcripts, however, also are expressed in many other tissues, including aorta, adrenal gland, bladder, brain, cerebellum, breast, heart, ileum, liver, lung, skeletal muscle, ovary, placenta, skin, spleen, stomach, uterus, and testes. The major transcript in most tissues is 2.3-2.5 kilobases in size. At least two larger mRNAs are observed in kidney and liver, and smaller transcripts are found in kidney, skin, and testes. The most abundant testicular transcript is 1.4-1.5 kilobases in size, and it hybridizes with two different cDNA probes that encode portions of the receptor sequence from the putative fourth transmembrane domain to its C-terminal end. It does not hybridize, however, with a probe encoding the first 107 residues of the receptor sequence. Although, PTH/PTHrP receptor mRNAs are highly expressed in kidney and bone, classic PTH targets that are associated with calcium homeostasis, their wide tissue distribution suggests that PTH and/or PTHrP have other physiological roles, particularly in these other tissues. The mechanisms leading to tissue-specific expression of PTH/PTHrP receptor transcripts of different sizes and the functions of these mRNAs remain to be determined.