Disruption of type 3 adenylyl cyclase expression in the hypothalamus leads to obesity.
Disruption of type 3 adenylyl cyclase expression in the hypothalamus leads to obesity.
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DOI：
10.15761/iod.1000149
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Storm DR
中科院分区：
文献类型：
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作者：
Cao H;Chen X;Yang Y;Storm DR
来自人类研究和缺乏3型腺苷酸环化酶（AC 3）的转基因小鼠的证据表明，AC 3在体重调节中起作用。目前尚不清楚AC 3在哪个大脑区域发挥这种作用。我们研究了AC 3在下丘脑中的作用，使用floxed AC 3小鼠品系控制体重。在这里，我们报告了AC 3 flox/flox小鼠在下丘脑中施用AAV-CRE-GFP后变得肥胖。雄性和雌性AC 3 floxed小鼠都显示出比注射AAV-GFP的对照小鼠更重的体重。此外，选择性消融下丘脑腹内侧AC 3表达的小鼠也显示体重和摄食量增加。我们的研究结果表明，下丘脑中的AC 3调节能量平衡。
Evidence from human studies and transgenic mice lacking the type 3 adenylyl cyclase (AC3) indicates that AC3 plays a role in the regulation of body weight. It is unknown in which brain region AC3 exerts such an effect. We examined the role of AC3 in the hypothalamus for body weight control using a floxed AC3 mouse strain. Here, we report that AC3 flox/flox mice became obese after the administration of AAV-CRE-GFP into the hypothalamus. Both male and female AC3 floxed mice showed heavier body weight than AAV-GFP injected control mice. Furthermore, mice with selective ablation of AC3 expression in the ventromedial hypothalamus also showed increased body weight and food consumption. Our results indicated that AC3 in the hypothalamus regulates energy balance.