Neonatal maternal separation causes decreased numbers of Sertoli cell, spermatogenic cells, and sperm in mic
Neonatal maternal separation causes decreased numbers of Sertoli cell, spermatogenic cells, and sperm in mic
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新生儿母体分离导致小鼠支持细胞、生精细胞和精子数量减少
DOI：
10.1080/15376516.2020.1841865
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Itoh M
中科院分区：
文献类型：
--
作者：
Miyaso H;Nagahori K;Takano K;Omotehara T;Kawata S;Li ZL;Kuramasu M;Wu X;Ogawa Y;Itoh M
新生儿母亲分离是一种实验模型，用于评估新生儿毒性应激或早期生活应激的影响。虽然在儿童时期的各种生理和心理压力已被报道，新生儿母亲分离对男性生殖系统的影响仍然不清楚。因此，本研究评估了新生儿母亲分离对男性生殖系统的影响。在新生雄性ICR小鼠中，从出生后第1天至第10天，每天进行0.5、1、2和4小时的母体分离。在10周龄时，新生的母体分离小鼠表现出睾丸重量和附睾精子数量的减少，沿着各种睾丸形态学变化，包括生殖细胞、支持细胞和间质细胞。值得注意的是，新生的母体分离小鼠显示支持细胞数量减少。动物进行0.5-，1-，和2-h/天的新生儿母亲分离表现出血清睾酮水平下降，但在促性腺激素（促黄体激素和卵泡刺激素）。总之，这些数据表明，雄性小鼠的新生儿母体分离导致青春期后支持细胞数量减少，导致随后的生精活性降低。
Neonatal maternal separation is an experimental model used to evaluate the effects of toxic stress in neonates, or early life stress. Although various physiological and psychological stresses during childhood have been reported, the effects of neonatal maternal separation on the male reproductive system remain unclear. Therefore, the present study evaluated the effects of neonatal maternal separation on the male reproductive system. In neonatal male ICR mice, maternal separation was performed for 0.5, 1, 2, and 4 hours/day, from postnatal day 1 to 10. At 10 weeks of age, the neonatal maternal separation mice exhibited decreases in both testicular weight and epididymal sperm number, along with various testicular morphological changes involving germ cells, Sertoli cells, and interstitial cells. Notably, neonatal maternal separation mice showed decreased numbers of Sertoli cells. Animals subjected to 0.5-, 1-, and 2-h/day neonatal maternal separation exhibited decreases in serum levels of testosterone but not in those of gonadotropin (luteinizing hormone and follicle-stimulating hormone). Together, these data showed that neonatal maternal separation in male mice causes decreased Sertoli cell numbers following puberty, resulting in subsequent decreased spermatogenic activity.