Bacterial probiotic modulation of dendritic cells
Bacterial probiotic modulation of dendritic cells
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DOI：
10.1128/iai.72.6.3299-3309.2004
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发表时间：
2004-06-01
影响因子：
3.1
通讯作者：
Czinn, S
中科院分区：
文献类型：
--
作者：
Drakes, M;Blanchard, T;Czinn, S
肠树突状细胞持续暴露于摄入的微生物和高浓度的内源性细菌植物群。这些细胞可以被感染因子和其他刺激物激活，以诱导T细胞应答并产生趋化因子，该趋化因子将其他细胞募集到局部环境中。细菌益生菌越来越多地用于治疗肠道疾病，如炎症性肠病。它们在肠道内作为非致病性刺激物，以恢复免疫静止。本研究旨在确定细菌益生菌混合物VSL#3改变骨髓来源的树突状细胞富集群体中细胞表面抗原表达和细胞因子产生的能力。单克隆荧光抗体染色监测细胞表面表型，酶联免疫吸附测定定量细胞因子水平。高剂量益生菌上调C80、CD 86、CD 40和主要组织相容性复合物II类I-A的表达（d）。在低剂量益生菌或干酪乳杆菌处理后，B7-DC或B7 RP-1均未增加，但B7 RP-1在革兰氏阴性细菌大肠杆菌刺激的树突状细胞上显示出增加的表达。功能研究表明，益生菌并不能增强树突状细胞诱导同种异体T细胞增殖的能力，如在大肠杆菌中观察到的那样。杆菌益生菌刺激3天后，在富含树突状细胞的培养上清液中观察到白细胞介素-10释放的显著增强。这些结果表明，益生菌具有调节树突状细胞表面表型和粒细胞-巨噬细胞集落刺激因子刺激的骨髓来源的树突状细胞中的细胞因子释放的能力。益生菌对树突状细胞细胞因子的调节可能有助于这些分子在肠道疾病治疗中的益处。
Intestinal dendritic cells are continually exposed to ingested microorganisms and high concentrations of endogenous bacterial flora. These cells can be activated by infectious agents and other stimuli to induce T-cell responses and to produce chemokines which recruit other cells to the local environment. Bacterial probiotics are of increasing use against intestinal disorders such as inflammatory bowel disease. They act as nonpathogenic stimuli within the gut to regain immunologic quiescence. This study was designed to determine the ability of a bacterial probiotic cocktail VSL#3 to alter cell surface antigen expression and cytokine production in bone marrow-derived dendritic cell-enriched populations. Cell surface phenotype was monitored by monoclonal fluorescent antibody staining, and cytokine levels were quantitated by enzyme-linked immunosorbent assay. High-dose probiotic upregulated the expression of C80, CD86, CD40, and major histocompatibility complex class II I-A(d). Neither B7-DC or B7RP-1 was augmented after low-dose probiotic or Lactobacillus casei treatment, but B7RP-1 showed increased expression on dendritic cells stimulated with the gram-negative bacterium Escherichia coli. Functional studies showed that probiotic did not enhance the ability of dendritic cells to induce allogeneic T-cell proliferation, as was observed for E. coli. Substantial enhancement of interieukin-10 release was observed in dendritic cell-enriched culture supernatants after 3 days of probiotic stimulation. These results demonstrate that probiotics possess the ability to modulate dendritic cell surface phenotype and cytokine release in granulocyte-macrophage colony-stimulating factor-stimulated bone marrow-derived dendritic cells. Regulation of dendritic cell cytokines by probiotics may contribute to the benefit of these molecules in treatment of intestinal diseases.