Synthesis of retinoic acid by rat ovarian cells that express cellular retinoic acid-binding protein-II.
Synthesis of retinoic acid by rat ovarian cells that express cellular retinoic acid-binding protein-II.
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表达细胞视黄酸结合蛋白-II 的大鼠卵巢细胞合成视黄酸。
DOI：
10.1095/biolreprod60.1.110
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发表时间：
1999
影响因子：
3.6
通讯作者：
Ong,DE
中科院分区：
文献类型：
--
作者：
Zheng,WL;Bucco,RA;Sierra-Rievera,E;Osteen,KG;Melner,MH;Ong,DE
大鼠注射eCG诱导假性孕激素后，48 h内卵巢颗粒细胞和子宫内膜上皮细胞中出现细胞视黄酸结合蛋白II（CRABP[II]）。这种表达模式也见于正常成熟雌性大鼠，其中CRABP（II）在发情期（但不在间情期）的子宫上皮中以及在卵巢的颗粒细胞和黄体细胞中表达。我们以前已经证明，子宫上皮细胞从假孕大鼠获得了合成视黄酸的能力，从视黄醇，在相关的诱导表达的CRABP（II）。如果这是真的CRABP（II）表达的其他网站，那么当地生产的视黄酸是密切相关的各个阶段的女性生殖。在这里，我们报告说，颗粒细胞从eCG处理的未成熟大鼠的卵巢和黄体细胞从eCG/hCG处理的未成熟大鼠的卵巢（两者都表达CRABP[II]）合成显着更高量的视黄酸培养时，与对照车辆处理的青春期前大鼠的卵巢培养的颗粒细胞相比。培养对照组或hCG治疗组的颗粒细胞对CRABP（II）细胞的表达没有影响。这些数据与我们的假设，CRABP（II）的表达与视黄酸的合成和加强的情况下，当地产生的视黄酸在生殖中起着重要的作用是一致的。
The induction of pseudopregnancy by the injection of eCG in rats results in the appearance of cellular retinoic acid-binding protein type II (CRABP[II]) in the granulosa cells of the ovary and the lining epithelium of the uterus within 48 h. This expression pattern is also seen in the normal mature female rat, in which CRABP(II) is expressed in the uterine epithelium during estrus (but not diestrus) and in the granulosa and luteal cells of the ovary. We have previously demonstrated that the uterine epithelial cells from the pseudopregnant rat have gained the ability to synthesize retinoic acid from retinol, in correlation with the induced expression of CRABP(II). If this is true for other sites of CRABP(II) expression, then local production of retinoic acid is intimately connected with various stages of reproduction in the female. Here we report that granulosa cells from the ovary of the eCG-treated immature rat and luteal cells from the ovary of the eCG/hCG-treated immature rat (both of which express CRABP[II]) synthesized markedly higher amounts of retinoic acid when cultured, compared to granulosa cells cultured from the ovary of the prepubertal rat treated with control vehicle. Culturing the granulosa cells from either control or eCG-treated animals had no effect on the expression of CRABP(II) cells. These data are consistent with our hypothesis that CRABP(II) expression is associated with retinoic acid synthesis and strengthen the case that local generation of retinoic acid plays an important role in reproduction.