CD14, A RECEPTOR FOR COMPLEXES OF LIPOPOLYSACCHARIDE (LPS) AND LPS BINDING-PROTEIN
CD14, A RECEPTOR FOR COMPLEXES OF LIPOPOLYSACCHARIDE (LPS) AND LPS BINDING-PROTEIN
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DOI：
10.1126/science.1698311
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发表时间：
1990-09-21
期刊：
影响因子：
56.9
通讯作者：
MATHISON, JC
中科院分区：
文献类型：
--
作者：
WRIGHT, SD;RAMOS, RA;MATHISON, JC
白细胞对每毫升纳克浓度的脂多糖（LPS）产生反应，分泌肿瘤坏死因子-α (TNF-α)等细胞因子。过量分泌TNF-α可引起内源性休克，这是感染的致命并发症。血液中的LPS迅速与血清蛋白脂多糖结合蛋白（LBP）结合，细胞对LPS生理水平的反应依赖于LBP。CD14是一种单核细胞分化抗原，可结合LPS和LBP复合物，单克隆抗体阻断CD14可阻止LPS培养的全血合成TNF-α。因此，LPS可能通过与血清中的可溶性结合蛋白相互作用诱导反应，然后结合细胞表面蛋白CD14。
Leukocytes respond to lipopolysaccharide (LPS) at nanogram per milliliter concentrations with secretion of cytokines such as tumor necrosis factor-α (TNF-α). Excess secretion of TNF-α causes endotoxic shock, an often fatal complication of infection. LPS in the bloodstream rapidly binds to the serum protein, lipopolysaccharide binding protein (LBP), and cellular responses to physiological levels of LPS are dependent on LBP. CD14, a differentiation antigen of monocytes, was found to bind complexes of LPS and LBP, and blockade of CD14 with monoclonal antibodies prevented synthesis of TNF-α by whole blood incubated with LPS. Thus, LPS may induce responses by interacting with a soluble binding protein in serum that then binds the cell surface protein CD14.