CHARACTERIZATION OF THE ALPHA-TUBULIN GENE FAMILY OF ARABIDOPSIS-THALIANA
CHARACTERIZATION OF THE ALPHA-TUBULIN GENE FAMILY OF ARABIDOPSIS-THALIANA
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DOI：
10.1073/pnas.84.16.5833
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发表时间：
1987-08-01
影响因子：
11.1
通讯作者：
SNUSTAD, DP
中科院分区：
文献类型：
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作者：
LUDWIG, SR;OPPENHEIMER, DG;SNUSTAD, DP
拟南芥基因组Heynhold被证明含有α-微管蛋白基因家族，该家族至少由4个基因和/或假基因组成。测定了转录的α-微管蛋白基因的一级结构。将拟南芥的α-微管蛋白的预测氨基酸序列与莱茵衣藻、花柱甲藻和智人的α-微管蛋白的预测氨基酸序列进行比较，发现高度同源性；分别为90%、87%和83%。因此，植物α-微管蛋白与原生动物和动物的α-微管蛋白具有高度的同源性。拟南芥α-微管蛋白基因的编码序列被四个内含子打断，这四个内含子出现在与莱茵哈迪尔锥虫和大鼠α-微管蛋白基因中数量较少的内含子不同的位置。S4核酸酶定位结果表明，该基因的转录启动为99.+-。翻译起始密码子上游1个碱基对。使用5‘’和3‘’非编码基因特异性探针，通过与从叶、根和花中提取的总RNA杂交来检测α-微管蛋白基因在这些组织中的表达。结果表明，α-微管蛋白基因在三种组织中均有转录。
The genome of Arabidopsis thaliana (Linnaeus) Heynhold was shown to contain an .alpha.-tubulin gene family consisting of at least four genes and/or pseudogenes. The primary structure of a transcribed .alpha.-tubulin gene was determined. A comparison of the predicted amino acid sequence of the A. thaliana .alpha.-tubulin with the predicted amino acid sequences of .alpha.-tubulins of Chlamydomonas reinhardtii, Stylonychia lemnae, and Homo sapiens reveals a high degree of homology; 90%, 87%, and 83% identity, respectively. Thus, a plant .alpha.-tubulin exhibits a high degree of homology to the .alpha.-tubulins of protists and animals. The coding sequence of the A. thaliana .alpha.-tubulin gene is interrupted by four introns, which occur at positions different from those of the less numerous introns of C. reinhardtii and rat .alpha.-tubulin genes. S4 nuclease mapping data showed that transcription is initiated 99 .+-. 1 base pairs upstream from the translation initiation codon. Both 5'' and 3'' noncoding gene-specific probes were used to examine the expression of the .alpha.-tubulin gene in leaves, roots, and flowers by hybridization to total RNA isolated from these tissues. The results showed that the .alpha.-tubulin gene was transcribed in all three tissues.