The tertiary structure of azurin from Pseudomonas denitrificans as determined by Cu resonant diffraction using synchrotron radiation.
The tertiary structure of azurin from Pseudomonas denitrificans as determined by Cu resonant diffraction using synchrotron radiation.
复制标题
使用同步辐射通过铜共振衍射测定脱氮假单胞菌中天青蛋白的三级结构。
DOI：
10.1016/0022-2836(87)90701-7
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发表时间：
1987
影响因子：
5.6
通讯作者：
Korszun,ZR
中科院分区：
文献类型：
--
作者：
Korszun,ZR
采用同步辐射薄膜数据采集方法，以3.0 Å分辨率解析了反硝化假单胞菌的结构。使用8.98 keV辐射收集Bijvoet对反射，其中Δf '和Δf″对azurin的Cu假体基散射的实校正和虚校正分别很大，而使用8.00 keV辐射收集的Bijvoet对反射的校正较小。利用差值帕特森合成法确定了Cu原子的位置，并对所观察到的蛋白质结构因子模量进行了相位分析。使用有限的数据集建立了蛋白质的原子模型，随后从初始模型计算了所有观察到的3.0 Å分辨率反射的相位。随后使用所有观测到的数据重建原子模型。本文介绍了这项工作的结果，并说明了同步辐射相位技术在解决金属蛋白结构方面的应用，而无需求助于多重同构替换。
The structure ofPseudomonas denitrificansazurin has been solved at 3.0 Å resolution by film data collection methods using synchrotron radiation. Bijvoet pairs of reflections were collected using 8.98 keV radiation where both Δf′ and Δf″, the real and imaginary corrections to azurin's Cu prosthetic group scattering, respectively, are large, and using 8.00 keV radiation where these corrections are smaller. The Cu atom was located by difference Patterson syntheses and used to phase the observed protein structure-factor moduli of selected reflections. An atomic model of the protein was built using a restricted data set and phases for all observed reflections to 3.0 Å resolution were subsequently calculated from the initial model. The atomic model was subsequently rebuilt using all observed data. The results of this work are presented here and illustrate the utility of synchrotron radiation phasing techniques in solving the structures of metalloproteins without recourse to multiple isomorphous replacement.