Aquaporin-1 expression in fluoro-edenite-induced mesothelioma effusions: An approach by cell-block procedure
Aquaporin-1 expression in fluoro-edenite-induced mesothelioma effusions: An approach by cell-block procedure
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DOI：
10.1111/cyt.12583
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发表时间：
2018-10-01
期刊：
影响因子：
1.3
通讯作者：
Tuccari, G.
中科院分区：
文献类型：
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作者：
Angelico, G.;Ieni, A.;Tuccari, G.
水通道蛋白1（Aquaporin 1，AQP-1）是一种存在于细胞膜上的水通道蛋白，其表达被认为是胸膜恶性间皮瘤（malignant mesothelioma，MM）的独立预后因子。本研究的目的是评估AQP-1的表达及其预后价值的一系列的MM胸腔积液，从一个地理区域的高浓度的氟-edenite（FE）。MethodsWe选择了25 MM的情况下，Biancavilla（意大利），一个地区的FE环境浓度高。所有病例的细胞学样本、细胞块（CB）、临床和随访数据均可用。免疫组化calretinin，CK 5/6，WT 1，CK 7和TTF 1 CB部分，以确认MM的细胞学诊断。免疫组化AQP-1进行和高表达定义时，50%的肿瘤细胞呈线性和圆周膜染色。ResultsThe队列包括16名男性和9名女性（中位年龄：67.5岁;范围：49- 88岁）。中位生存期14个月（1.5 ~ 60个月），差异有统计学意义（P = 0.006）。所有病例均经组织学证实，分为上皮样型（16例）、双相型（7例）和肉瘤样型（2例）。AQP-1高表达16例。比较AQP-1高表达与相应患者的生存率，已证实AQP-1高表达与12个月总生存率略微增加显著相关。9例AQP-1评分低于50%的患者中位总生存期较短（7个月）。结论AQP-1高表达可在FE诱导的MM细胞学样本中检测到，并具有预后价值。本研究首次评估了水通道蛋白-1（AQP-1）免疫表达在一组罕见的荧光检测患者胸腔积液中的诊断价值和预后意义。胸膜恶性间皮瘤。
ObjectiveAquaporin 1 (AQP-1) is a water channel protein found in cell membranes, whose expression has been considered an independent favourable prognostic factor in pleural malignant mesothelioma (MM). The aim of this study was to evaluate the expression of AQP-1 and its prognostic value in a series of pleural MM effusions, from a geographical area with high concentrations of fluoro-edenite (FE).MethodsWe selected 25 MM cases from Biancavilla (Italy), an area with high environmental concentrations of FE. Cytological samples, cell-blocks (CB), clinical and follow-up data were available for all cases. Immunohistochemistry for calretinin, CK5/6, WT1, CK7 and TTF1 was used on CB sections to confirm the cytological diagnosis of MM. Immunohistochemistry for AQP-1 was performed and high expression was defined when 50% of tumour cells showed linear and circumferential membranous staining.ResultsThe cohort included 16 men and nine women (median age: 67.5years; range: 49-88years). The median survival was 14months (range 1.5-60months), with a significant value (P = 0.006). All cases have been histologically confirmed and classified as epithelioid (16 cases), biphasic (seven cases) and sarcomatoid (two cases). AQP-1 high expression has been observed in 16 cases. Comparing AQP-1 high expression to the survival of corresponding patients, a significant association with a slight increased overall survival of 12months has been demonstrated. Nine patients with a AQP-1 score less than 50% showed a shorter median overall survival (7months).ConclusionsAQP-1 high expression is detectable on cytological samples of FE-induced MM with a prognostic value.This study presents the first evaluation of the diagnostic usefulness and prognostic implications of aquaporin-1 (AQP-1) immunoexpression in pleural effusions of a rare group of patients with Fluoro-Edenite induced pleural malignant mesothelioma.