Monoclonal antibodies cross-reactive with group A streptococci and normal and psoriatic human skin.
Monoclonal antibodies cross-reactive with group A streptococci and normal and psoriatic human skin.
复制标题
单克隆抗体与 A 组链球菌以及正常和银屑病人类皮肤发生交叉反应。
DOI：
10.1111/1523-1747.ep12524838
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Hall,NK
中科院分区：
文献类型：
--
作者：
Swerlick,RA;Cunningham,MW;Hall,NK
A群链球菌感染已被认为是银屑病恶化的一个因素。为了探讨这种现象中链球菌抗原与人体皮肤交叉反应的可能性，我们用3种抗A组链球菌单克隆抗体与银屑病患者和对照组皮肤反应，并采用间接免疫荧光技术估计抗体结合情况。单克隆抗体54.2.8染色表皮和表皮附属物内细胞的细胞核和细胞质，以及分散在真皮内的细胞。相比之下，49.8.2和36.2.2单克隆抗体能标记表皮细胞和表皮附属物的细胞质，但不与细胞核发生反应。银屑病患者对照皮肤和未受损伤皮肤的染色模式无差异。然而，银屑病皮损的皮肤含有大量的交叉反应性皮肤成分。与正常皮肤或银屑病患者的血清相比，点滴状银屑病患者的血清反应没有差异。皮肤提取物的Western免疫印迹表明，单克隆抗体54.2.8与分子量在60-70K范围内的蛋白家族发生反应。结果表明，人皮肤中的成分与A群链球菌具有相同的交叉反应表位。A组链球菌与人体皮肤之间的免疫交叉反应可能在链球菌感染后某些皮肤疾病的恶化中起重要作用。
Infection with group A streptococci has been implicated as a factor capable of exacerbating psoriasis. In order to explore the possibility of cross-reactivity between streptococcal antigens and human skin in this phenomenon, skin from psoriatic patients and control subjects was reacted with 3 monoclonal antibodies against group A streptococci and antibody binding was estimated by the indirect immunofluorecence technique. Monoclonal antibody 54.2.8 stained the nuclei and cytoplasm of cells within the epidermis and epidermal appendages, as well as cells scattered throughout the dermis. In contrast, monoclonal antibodies 49.8.2 and 36.2.2 labeled the cytoplasm of epidermal cells and epidermal appendages but did not react with nuclei. No difference in the staining patterns of control skin and uninvolved skin from patients with psoriasis was observed. However, skin from psoriatic lesions contained large amounts of cross-reactive skin component(s). Sera from patients with guttate psoriasis did not react differently with normal or psoriatic skin when compared with normal sera. Western immunoblots of skin extracts demonstrated that monoclonal antibody 54.2.8 reacted with a family of proteins in the molecular weight range of 60–70K. The results indicate that component(s) in human skin share cross-reactive epitopes with group A streptococci. Immunologic cross-reactions between group A streptococci and human skin may play an important role in the exacerbation of certain skin disorders following streptococcal infections.