Effects of Botox® and Neuronox® on muscle force generation in mice
Effects of Botox® and Neuronox® on muscle force generation in mice
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DOI：
10.1002/jor.20450
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发表时间：
2007-12-01
影响因子：
2.8
通讯作者：
Callahan, Michael F.
中科院分区：
文献类型：
--
作者：
Stone, Austin V.;Ma, Jianjun;Callahan, Michael F.
目前的研究确定了两种市售 A 型肉毒杆菌神经毒素 (BoNTA) 制剂（Botox(R) 和 Neuronox(R)）在小鼠模型中抑制肌肉力量的剂量-反应关系，并描述了毒素引起的肌肉麻痹恢复的时间过程。还确定了冷冻重构毒素对毒素效力的影响。雄性 CD-1 小鼠的腓肠肌注射生理盐水或 BoNTA (0.3-3.0 U/kg)，并在刺激胫神经（单次抽搐和 15-200 Hz 手足搐搦）后检查肌肉力量的产生。注射毒素 4 天后，Botox 和 Neuronox 产生几乎相同的肌肉力量下降 (30%-90%)。注射（1 U/kg）后 28 天，肌肉力量从两种毒素制剂的影响中恢复。将重构的毒素在 -80 摄氏度下保存长达 5 个月，并没有导致毒素活性显着丧失。这项研究的结果表明，Botox 和 Neuronox 在小鼠模型中产生相同的反应，并且与其他模型相比，肌肉恢复速度很快，毒素剂量产生的肌肉力量小于最大减量。 (C) 2007 年骨科研究学会。
The current study determined the dose-response relationship for inhibition of muscle force of two commercially available botulinum neurotoxin type-A(BoNTA) preparations (Botox(R) and Neuronox(R)) in a murine model and characterized the time course of recovery from the toxin-induced muscle paralysis. The effect of freezing reconstituted toxin on toxin potency was also determined. The gastrocnemius muscles in male CD-1 mice were injected with either saline or BoNTA (0.3-3.0 U/kg), and muscle force generation was examined following stimulation of the tibial nerve (single twitch and 15-200 Hz tetany). Botox and Neuronox produced nearly equivalent decrements in muscle force (30%-90%) at 4 days after toxin injection. At 28 days after injection (1 U/kg), muscle force had recovered from the effects of both toxin preparations. Maintaining reconstituted toxin at -80 degrees C for up to 5 months did not result in significant loss of toxin activity. The results of this study suggest that Botox and Neuronox produce equivalent responses in a murine model, and, in contrast to other models, muscle recovery is rapid with doses of toxin that produce less than maximal decrements in muscle force. (C) 2007 Orthopaedic Research Society.