Biosensor arrays for simultaneous measurement of glucose, lactate, glutamate, and glutamine
Biosensor arrays for simultaneous measurement of glucose, lactate, glutamate, and glutamine
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DOI：
10.1016/s0956-5663(01)00298-6
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发表时间：
2002-04-01
影响因子：
12.6
通讯作者：
Urban, GA
中科院分区：
文献类型：
--
作者：
Moser, I;Jobst, G;Urban, GA
为了同时测量葡萄糖、乳酸、谷氨酰胺和谷氨酸，在微流系统中实现了生物传感器阵列，从而给出了微系统。该微系统由集成了生物传感器阵列的玻璃芯片和底部组成，底部由金反电极、300米厚的密封和电气互连线组成。当与芯片上的混合器集成时，流动装置的总内部体积为2.1或6摩尔。利用光致型酶膜对电化学传感器进行修饰，制备出无串扰、长期稳定的生物传感器。适当的小型化技术的使用导致在全血和发酵液中用于体内和体外应用的可批量生产的设备。由于一种新的谷氨酰胺酶在中性pH范围内具有最佳活性，因此可以直接和同时监测谷氨酰胺以及葡萄糖，乳酸和谷氨酸。(C) 2002 Elsevier Science B.V.版权所有
For simultaneous measurement of glucose, lactate, glutamine, and glutamate a biosensor array is implemented in a micro flow-system thus giving a microsystem. The microsystem consists of a glass chip with the integrated biosensor array and a bottom part, which comprises a gold counter electrode, a 300 m thick seal, and electrical interconnection lines. The flow device has a total internal volume of 2.1 or 6 mul when integrated with a mixer on chip. The biosensors with no crosstalking and high long term stability were produced by modifying the electrochemical transducers and utilizing photopatternable enzyme membranes. The use of appropriate miniaturization technology leads to mass producable devices for in vivo and ex vivo applications in whole blood and fermentation broth. Due to a novel glutaminase with an activity optimum in the neutral pH range direct and simultaneous monitoring of glutamine together with glucose, lactate, and glutamate could be performed. (C) 2002 Elsevier Science B.V. All rights reserved.