VARIABLE EXPRESSION OF Ag‐B AND NON‐Ag-B HISTOCOMPATIBILITY ANTIGENS ON CULTURED RAT CELLS OF DIFFERENT HISTOLOGICAL ORIGIN
VARIABLE EXPRESSION OF Ag‐B AND NON‐Ag-B HISTOCOMPATIBILITY ANTIGENS ON CULTURED RAT CELLS OF DIFFERENT HISTOLOGICAL ORIGIN
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Ag-B 和非 Ag-B 组织相容性抗原在不同组织学来源的培养大鼠细胞上的可变表达
DOI：
10.1097/00007890-197310000-00006
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发表时间：
1973
期刊：
影响因子：
6.2
通讯作者：
J. Palm
中科院分区：
文献类型：
--
作者：
S. J. Hausman;J. Palm
用混合血凝试验（MHA）检测不同年龄大鼠不同组织器官培养细胞中几种组织相容性抗原的存在。由大鼠主要组织相容性位点确定的抗原表达的时间或组织学变化，表现为来自胚胎和成人组织的细胞系。在新生儿脑、皮肤、肝脏、心脏、眼睛、脾脏和肾脏的细胞原代培养物上检测到Ag-B抗原。肾源性培养物的一个特点是存在不与任何测试的抗血清（包括异源兔抗大鼠血清）反应的上皮细胞斑块。非Ag-B抗原，包括男性相关抗原，也用MHA检测到。这些抗原存在于培养的组织细胞系上，但不存在于除皮肤外的任何器官来源的培养物上。皮肤培养的上皮样细胞，但不是成纤维细胞，是反应性的。胶原介导的掩蔽现象，显着降低或climeded非Ag-B抗血清的反应性也被揭示。
Cells cultured from different tissues and organs of various aged rats were tested by the mixed hemagglutination assay (MHA) for the presence of several histocompatibility antigens. Chronological or histological variations in expression of antigens determined by the major histocompatibility locus of the rat, Ag-B, were exhibited by cell lines derived from both embryonic and adult tissues. Ag-B antigens were detected on primary cultures of cells from neonatal brain, skin, liver, heart, eye, spleen, and kidney. A peculiarity of the kidney-derived cultures was the presence of patches of epithelial cells which did not react with any antiserum tested, including a heterologous rabbit antirat serum. Non-Ag-B antigens, including a male-associated antigen, were also detected with MHA. These antigens were present on cultured tissue cell lines, but not on any of the organ-derived cultures, except skin. The epithelial-like cells of skin cultures, but not the fibroblasts, were reactive. A collagen-mediated masking phenomenon which markedly reduced or climinated reactivity of non-Ag-B antisera was also revealed.