The process of otoconia formation in guinea pig utricular supporting cells.
The process of otoconia formation in guinea pig utricular supporting cells.
复制标题
豚鼠椭圆囊支持细胞耳石形成的过程。
作者：
Y. Harada;S. Kasuga;N. Mori
为了阐明耳郭形成的机制，我们研究了钙在耳郭支持细胞中的转运途径。焦锑酸钾（PA）沉淀不仅存在于耳郭中，也存在于支持细胞的细胞质中，表明钙离子的定位。后者在分泌颗粒和内质网（ER）中检测到PA沉积。在用乙二醇-O，O，-双（2-氨基乙基）-N，N，N‘，N’-四乙酸（EGTA）预处理的细胞中不存在这些沉积物。支持细胞暴露于硫酸链霉素（SM）中增加了溶酶体的数量。这些溶酶体含有许多小的PA沉积物。细胞质中剩余的颗粒和内质网也含有少量PA沉积。提示耳郭是由前庭支持细胞形成的，钙离子可能通过er分泌颗粒-溶酶体-细胞质突起转运。
In order to clarify the mechanism of otoconia formation, the pathway of calcium transport in the utricular supporting cells was investigated. Potassium pyroantimonate (PA) precipitation, which indicates the localization of calcium ions, was seen not only in otoconia but also in supporting cell cytoplasm. In the latter, deposits of PA were detected in the secretory granules and endoplasmic reticulum (ER). These deposits were not present in cells pretreated with ethylene glycol-O,O,-bis (2-aminoethyl)-N,N,N',N'-tetra-acetic acid (EGTA). Exposure of the supporting cells to streptomycin sulfate (SM) increased the number of lysosomes. These lysosomes contained many small deposits of PA. The remaining granules and ER in cytoplasm also contained small deposits of PA. The findings suggest that otoconia are formed by the vestibular supporting cells in which calcium ions might be transported via ER-secretory granule-lysosome-cytoplasmic protrusion.