Female control of paternity in the internally fertilizing compound ascidian Diplosoma listerianum. II. Investigation of male mating success using RAPD markers
Female control of paternity in the internally fertilizing compound ascidian Diplosoma listerianum. II. Investigation of male mating success using RAPD markers
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体内受精复合海鞘 Diplosoma listerianum 中雌性对父子关系的控制。
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
L. Noble
中科院分区：
文献类型：
--
作者：
J. Bishop;Catherine S. Jones;L. Noble
随机扩增多态性DNA亲子标记被用来评估男性成功的同时，三路交配的机会之间的培养克隆的雌雄同体的原脊索动物Diplosomaisrianum。先前报道的精子在输卵管中运动的阻塞，阻止进入卵巢中的受精部位，被证明可以防止两个特定克隆之间的交叉受精。同样的机制可以防止自交。直接观察一组更广泛的实验室克隆来自同一个自然种群的配对表明，失败的一个或两个合作伙伴对产生育雏后代是比较常见的。一个弱的负相关性之间的交配成功的对和他们的整体遗传相似性估计从RAPD指纹从几个引物。这些数据表明，在D。listerianum.这可能涉及输卵管中与母体组织共享自我识别标记的精子的差异性吞噬清除。
Randomly amplified polymorphic DNA paternity markers were used to assess male success during simultaneous, three-way mating opportunities between cultured clones of the hermaphroditic protochordate Diplosoma listerianum. The previously reported blockage of sperm movement in the oviduct, barring access to the site of fertilization in the ovary, was shown to prevent cross-fertilization between two particular clones. The same mechanism prevents selfing. Direct observation of pairings within a more extensive set of laboratory clones derived from the same natural population indicated that failure of one or both partners of a pair to produce brooded progeny was relatively common. A weak negative correlation was found between the mating success of pairs and their overall genetic similarity estimated from RAPD fingerprints from several primers. The data suggest the operation of a somatic-gametic incompatibility mechanism regulating mating in D. listerianum. This may involve differential phagocytic removal, in the oviduct, of sperm that share self-recognition markers with maternal tissue.
使用 RAPD PCR 检测已知灵长类谱系后代的非亲本条带过多。
影响因子：
14.9
作者：
Riedy,MF;Hamilton3rd,WJ;Aquadro,CF
通讯作者：
Aquadro,CF