Validation of a new species for studying postoperative atrial fibrillation: Swine sterile pericarditis model.
Validation of a new species for studying postoperative atrial fibrillation: Swine sterile pericarditis model.
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验证用于研究术后心房颤动的新物种：猪无菌心包炎模型。
DOI：
10.1111/pace.14765
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Waldo,Albert
中科院分区：
文献类型：
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作者：
Lee,Seungyup;Khrestian,Celeen;Laurita,Daniel;Juzbasich,Dragan;Wallick,Don;Waldo,Albert
研究背景犬无菌性心包炎伴心房炎症模型是术后心房颤动（POAF）的实验模型。然而，使用犬进行研究在许多国家受到伦理委员会的限制，社会接受度正在下降。目的验证猪无菌性心包炎模型作为研究POAF的实验对应物的可行性。方法7只家猪（35-60 kg）进行初次心包炎手术。在术后两天或两天以上的闭胸状态下，我们在从右心耳（RAA）和左心房后壁（PLA）起搏时进行了起搏阈值和心房有效不应期（AERP）的电生理测量。在清醒和麻醉闭胸状态下测定短阵快速起搏诱发POAF（>5 min）。这些数据进行了比较，以前发表的犬无菌性心包炎的数据validation.ResultsThe起搏阈值增加，从第1天到第3天（2 ± 0.1至3.3 ± 0.6 mA的RAA，2.5 ± 0.1至4.8 ± 0.2 mA的PLA）。AERP也从第1天到第3天增加（RAA为118 ± 8到157 ± 16 ms，PLA为98 ± 4到124 ± 2 ms，两者均P <0.05）。持续性POAF的诱导发生在43%（POAF CL范围74-124 ms）。所有的电生理数据从猪模型是一致的犬模型方面（1）起搏阈值和AERP的范围;（2）阈值和AERP随着时间的推移进行性增加;（3）POAF的发病率为40%-50%。
BackgroundThe canine sterile pericarditis model associated with atrial inflammation is an experimental counterpart of postoperative atrial fibrillation (POAF). However, the use of canines for research is restricted by ethics committees in many countries, and social acceptance is declining.ObjectiveTo validate the feasibility of the swine sterile pericarditis model as an experimental counterpart to study POAF.MethodsSeven domestic pigs (35–60 kg) underwent initial pericarditis surgery. On two or more postoperative days in the closed‐chest state, we performed electrophysiological measurements of pacing threshold and atrial effective refractory period (AERP) while pacing from the right atrial appendage (RAA) and the posterior left atrium (PLA). The inducibility of POAF (>5 min) by burst pacing was determined in both the conscious and anesthetized closed‐chest state. These data were compared to previously published canine sterile pericarditis data for validation.ResultsThe pacing threshold increased from day 1 to day 3 (2 ± 0.1 to 3.3 ± 0.6 mA in the RAA, 2.5 ± 0.1 to 4.8 ± 0.2 mA in the PLA). Also, the AERP increased from day 1 to day 3 (118 ± 8 to 157 ± 16 ms in the RAA; 98 ± 4 to 124 ± 2 ms in the PLA, bothp< .05). Induction of sustained POAF occurred in 43% (POAF CL range 74–124 ms). All electrophysiologic data from the swine model were consistent with the canine model with respect to (1) the range of both pacing threshold and AERP; (2) the progressive increase in threshold and AERP over time; (3) a 40%–50% incidence of POAF.ConclusionA newly developed swine sterile pericarditis model demonstrated electrophysiologic properties consistent with the canine model and patients after open heart surgery.