Applicability of the glomerular size distribution coefficient in assessing human glomerular volume: the Weibel and Gomez method revisited
Applicability of the glomerular size distribution coefficient in assessing human glomerular volume: the Weibel and Gomez method revisited
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DOI：
10.1111/j.1469-7580.2007.00715.x
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发表时间：
2007-05-01
影响因子：
2.4
通讯作者：
Bertram, John F.
中科院分区：
文献类型：
--
作者：
Samuel, Terry;Hoy, Wendy E.;Bertram, John F.
肾小球体积（V-glom）的变化在各种肾小球疾病的发生和发展中起着重要作用。正常肾脏中V-glom的估计为疾病中肾小球肥大的研究提供了基线值。传统的基于模型的方法广泛应用于临床活检标本中的V-glom估计。该方法所需的肾小球大小分布和形状的假设是潜在的偏倚来源，尚未得到验证。我们评估了肾小球大小分布系数在估计人肾V-glom中的适用性。采用无偏的Disector/Cavalieri法对24名男性（20-69岁）的720个组织学正常的肾小球的V-glom进行了估计。精确的肾小球直径计算从Cavalieri估计的V-glom假设肾小球球形。将肾小球直径的变异系数（CV）与由Weibel和Gomez方法预测的尺寸分布系数的相应值进行比较。平均（SD）肾小球直径为201（28）mm（范围110-276 mm）。每个肾脏内肾小球直径的CV范围为4.9 - 14.6%。相应的肾小球大小分布系数预测的公式的韦伯和戈麦斯范围从1.00到1.03。当估计正常人肾脏中的V-glom时，由Weibel和Gomez技术所需的尺寸分布系数的值是显著恒定的。尽管V-glom有很大的变化。未来的研究应该检查这种方法的肾小球形状假设所引入的偏倚程度。
Changes in glomerular volume (V-glom) play an important role in the initiation and progression of various glomerulopathies. Estimation of V-glom in the normal kidney provides baseline values for studies of glomerular hypertrophy in disease. The traditional model-based method of Weibel and Gomez is widely applied to estimate V-glom in clinical biopsy specimens. Assumptions of glomerular size distribution and shape required by this method are potential sources of bias that have not been verified. We evaluated the applicability of the glomerular size distribution coefficient in estimating V-glom in human kidneys. V-glom of 720 non-sclerotic glomeruli in histologically normal kidneys of 24 males (20-69 years) was estimated by the unbiased disector/Cavalieri approach. Accurate glomerular diameters were calculated from Cavalieri estimates of V-glom assuming glomerular sphericity. The coefficients of variation (CV) of glomerular diameters were compared with the corresponding values of the size distribution coefficient predicted by the Weibel and Gomez method. Mean (SD) glomerular diameter was 201 (28) mm (range 110-276 mm). The CV of glomerular diameter within each kidney ranged from 4.9 to 14.6%. Corresponding glomerular size distribution coefficients predicted by the formula of Weibel and Gomez ranged from 1.00 to just 1.03. The value of the size distribution coefficient required by the Weibel and Gomez technique when estimating V-glom in normal human kidneys is remarkably constant. This is despite large variations in V-glom. Future studies should examine the extent of bias introduced by the glomerular shape assumptions of this method.