Antigenic, Immunologic and Genetic Characterization of Rough Strains B.abortus RB51, B.melitensis B115 and B.melitensis B18
Antigenic, Immunologic and Genetic Characterization of Rough Strains B.abortus RB51, B.melitensis B115 and B.melitensis B18
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DOI：
10.1371/journal.pone.0024073
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发表时间：
2011-10-31
期刊：
影响因子：
3.7
通讯作者：
Tarantino, Michela
中科院分区：
文献类型：
--
作者：
Adone, Rosanna;Muscillo, Michele;Tarantino, Michela
脂多糖（LPS）是布鲁氏菌的主要毒力因子。已经鉴定了几个基因参与三种LPS组分的合成：脂质A、核心和O-PS。通常，缺乏O-PS的布鲁氏菌菌株（粗糙突变体）的毒性低于野生型，并且不会诱导不需要的干扰抗体。这些药物在小鼠中被证明对布鲁氏菌病具有保护作用。由于这些有利的特征，粗糙菌株被认为是潜在的布鲁氏菌病疫苗。本研究对粗糙株B的抗原性、免疫学和遗传学特性进行了研究。abortus RB51、B. melitensis B115和B. Melitensis B18. RB 51来源于B。B115是一株天然粗糙型菌株，O-PS存在于细胞质中。B18是本实验室分离的一株利福平粗耐药突变株。通过测试RB 51、B115和B18与粗糙或光滑布鲁氏菌菌株诱导的抗体结合的能力来评价它们的表面抗原性。在家兔中评价了每种菌株诱导的抗体应答。对参与LPS合成的21个基因进行测序，并与B进行比较。melitensis 16 M菌株。结果表明，RB 51、B115和B18在抗原性、免疫学和遗传学特性上存在差异。特别是在B115中检测到wzm基因的无义突变，这可以解释O-PS在该菌株中的细胞内定位。互补研究，以评估每个突变的确切作用，影响布鲁氏菌的形态和毒力，可以提供有用的信息，评估新的，减毒疫苗的布鲁氏菌病。
The lipopolysaccharide (LPS) is considered the major virulent factor in Brucella spp. Several genes have been identified involved in the synthesis of the three LPS components: lipid A, core and O-PS. Usually, Brucella strains devoid of O-PS (rough mutants) are less virulent than the wild type and do not induce undesirable interfering antibodies. Such of them proved to be protective against brucellosis in mice. Because of these favorable features, rough strains have been considered potential brucellosis vaccines. In this study, we evaluated the antigenic, immunologic and genetic characteristics of rough strains B. abortus RB51, B. melitensis B115 and B. melitensis B18. RB51 derived from B. abortus 2308 virulent strain and B115 is a natural rough strain in which the O-PS is present in the cytoplasm. B18 is a rough rifampin-resistan mutant isolated in our laboratory. The surface antigenicity of RB51, B115 and B18 was evaluated by testing their ability to bind antibodies induced by rough or smooth Brucella strains. The antibody response induced by each strain was evaluated in rabbits. Twenty-one genes, involved in the LPS-synthesis, were sequenced and compared with the B. melitensis 16M strain. The results indicated that RB51, B115 and B18 have differences in antigenicity, immunologic and genetic properties. Particularly, in B115 a nonsense mutation was detected in wzm gene, which could explain the intracellular localization of O-PS in this strain. Complementation studies to evaluate the precise role of each mutation in affecting Brucella morphology and its virulence, could provide useful information for the assessment of new, attenuated vaccines for brucellosis.