Inhalation Exposure Method for Illegal Drugs.
Inhalation Exposure Method for Illegal Drugs.
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非法药物的吸入暴露方法。
DOI：
10.1248/yakushi.17-00019
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Y. Kohno
中科院分区：
文献类型：
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作者：
A. Inomata;A. Ogata;Y. Tada;A. Nagasawa;K. Yuzawa;H. Ando;Y. Kubo;Hiroshi Takahashi;Fujifumi Kaihoko;Kazuyoshi Tanaka;J. Nakajima;Atsuko Suzuki;Nozomi Uemura;T. Moriyasu;Daisuke Watanabe;Keirou Ishihara;Takashi Usami;Satoru Kamei;Y. Kohno
我们开发了一种新的吸入暴露方法来评估合成大麻仿制品的影响，这些仿制品正在东京地区作为新的，不受管制的药物分发。我们选择含有AB-CHMINACA和5F-AMB的商品“souou”作为试验药物，棉花糖（Althaea officinalis）干叶作为阴性对照。点燃半支装着干棉花糖叶或香烟叶的香烟，然后将每支香烟的主流烟雾送到一个暴露箱中的五只老鼠身上。香烟完全吸完后，在暴露后15、30和60分钟进行神经行为观察和麻痹测试。将暴露箱排出的空气倒入含有乙腈（第一冲击器）和二甲亚砜（第二冲击器）的冲击器中。对所得溶液进行分析，以评估合成大麻模拟物的分解情况。暴露于南头烟的小鼠在15、30和60 min表现出多种兴奋行为和一些抑制行为。这些显然包括大麻模拟的特定药理作用。阴性对照小鼠在15 min时也表现出一些抑制行为，但在随后的时间里这些行为逐渐减弱，在60 min时几乎消失。此外，在对照组中观察到的行为影响不如暴露在souou中的小鼠明显。在我们的研究中开发的吸入暴露方法将有效地确定非法药物的大麻素特异性药理作用，以及通过将测试物质与阴性对照进行比较来评估活性化合物的存在。
We developed a new inhalation exposure method to evaluate effects of synthetic cannabimimetics that are being distributed as new, unregulated drugs in the Tokyo area. We selected the commercial product "SOUTOU" containing AB-CHMINACA and 5F-AMB as the test drug and dried marshmallow (Althaea officinalis) leaves as the negative control. A half cigarette packed with dried marshmallow leaves or SOUTOU was ignited, then mainstream smoke from each was delivered to five mice in an exposure box. After the cigarettes were fully consumed, neurobehavioral observations and a catalepsy test were performed at 15, 30 and 60 min after exposure. The effluent air from the exposure box was poured into impingers containing acetonitrile (first impinger) and dimethyl sulfoxide (second impinger). The resulting solutions were analyzed to assess decomposition of the synthetic cannabimimetics. Mice exposed to SOUTOU smoke showed many excitement behaviors and some suppressive behaviors at 15, 30 and 60 min. These clearly included cannabimimetic specific pharmacological actions. Negative control mice also showed some suppressive behaviors at 15 min but these were attenuated at later times, nearly disappearing at 60 min. In addition, the behavioral effects observed in controls were less pronounced than those in SOUTOU exposed mice. The inhalation exposure method developed in our study would be effective for determining cannabinoid specific pharmacological effects of illegal drugs, as well as for assessing the presence of active compound(s) by comparing the test substance with a negative control.