Effect of phloroglucinol on oxidative stress and inflammation
Effect of phloroglucinol on oxidative stress and inflammation
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DOI：
10.1016/j.fct.2010.07.029
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发表时间：
2010-10-01
影响因子：
4.3
通讯作者：
Kim, Se-Kwon
中科院分区：
文献类型：
--
作者：
Kim, Moon-Moo;Kim, Se-Kwon
本研究旨在探讨间苯三酚对氧化应激和炎症的抑制作用。间苯三酚是褐藻中丰富的间苯单宁单体。间苯三酚的一些作用已被报道，但其抗氧化应激相关炎症的作用至今尚未有详细的报道。在我们的研究中，间苯三酚不仅对氧化应激有抑制作用，而且对脂多糖刺激RAW264.7细胞中肿瘤坏死因子-a、白细胞介素-1 β、白细胞介素-6和前列腺素E-2等炎症介质的产生也有抑制作用。此外，在HT1080细胞（一种人纤维肉瘤细胞系）中，间苯三酚降低了在慢性炎症中起重要作用的基质金属蛋白酶的表达。在间苯三酚存在的情况下，调节这些炎症介质表达的NF-kappa B转录因子通过抑制NIK而失活，AP-1转录因子的激活也通过抑制ERK（一种MAPK）而降低。这些结果表明间苯三酚可以为预防慢性炎症提供治疗潜力。(C) 2010 Elsevier Ltd.版权所有。
The aim of this study is to investigate the inhibitory effect of phloroglucinol on oxidative stress and inflammation. Phloroglucinol is a monomer of phlorotannins abundant in brown algae. A few effects of phloroglucinol have been reported, but any information on its effect on anti-inflammation related to oxidative stress has not been reported in detail until now. In our study phloroglucinol exhibited inhibitory effects not only on oxidative stress but also on the production of inflammatory mediators such as tumor necrosis factor-a, interleukin-1 beta, interleukin-6 and prostaglandin E-2 in RAW264.7 cells stimulated by lipopolysaccharide. Furthermore, in HT1080 cells, a human fibrosarcoma cell line, phloroglucinol decreased the expression of matrix metalloproteinase that play an important role in chronic inflammation. In the presence of phloroglucinol NF-kappa B transcription factor that regulates the expression of these inflammatory mediators was inactivated via inhibition of NIK, and activation of AP-1 transcription factor was also reduced via inactivation of ERK, a MAPK. These results suggest that phloroglucinol can provide a therapeutic potential for prevention of chronic inflammation. (C) 2010 Elsevier Ltd. All rights reserved.