ABSENCE OF TGF-BETA RECEPTORS AND GROWTH INHIBITORY RESPONSES IN RETINOBLASTOMA CELLS
ABSENCE OF TGF-BETA RECEPTORS AND GROWTH INHIBITORY RESPONSES IN RETINOBLASTOMA CELLS
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DOI：
10.1126/science.2895499
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发表时间：
1988-04-08
期刊：
影响因子：
56.9
通讯作者：
MASSAGUE, J
中科院分区：
文献类型：
--
作者：
KIMCHI, A;WANG, XF;MASSAGUE, J
检测视网膜母细胞瘤肿瘤细胞和正常视网膜细胞对各种生长抑制因子的反应。胎儿视网膜细胞对转化生长因子β 1（TGF-β 1）的抗有丝分裂作用高度敏感，而视网膜母细胞瘤肿瘤细胞系均对该因子具有抗性。用放射性碘标记的TGF-β 1对这些细胞进行结合测定和亲和标记，结果显示这些细胞不具有TGF-β 1。受体。视网膜母细胞瘤细胞缺乏通常在人类细胞系中看到的65，95和300千道尔顿的三种亲和标记蛋白，因此不同于正常视网膜细胞和显示正常受体模式的其他类型的神经外胚层肿瘤。TGF-β的损失受体是肿瘤细胞中罕见的事件，可能代表了这些细胞逃避阴性对照并形成视网膜母细胞瘤的一种机制。
The responses of retinoblastoma tumor cells and normal retinal cells to various growth inhibitory factors were examined. Whereas fetal retinal cells were highly sensitive to the antimitogenic effects of transforming growth factor .beta.1 (TGF-.beta.1), retinoblastoma tumor cell lines were all resistant to this factor. Binding assays and affinity labeling of these cells with radioiodinated TGF-.beta.1 revealed that the cells did not have TGF-.beta. receptors. The retinoblastoma cells lacked three affinity-labeled proteins of 65, 95, and 300 kilodaltons typically seen in human cell lines and thus differed from normal retinal cells and from other types of neuroectodermal tumors that display the normal pattern of receptors. Loss of TGF-.beta. receptors, which is a rare event among tumor cells, may represent one mechanism through which these cells escape from negative control and form retinoblastomas.