Intracellular glucose concentration in derepressed yeast cells consuming glucose is high enough to reduce the glucose transport rate by 50%
Intracellular glucose concentration in derepressed yeast cells consuming glucose is high enough to reduce the glucose transport rate by 50%
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DOI：
10.1128/jb.180.3.556-562.1998
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发表时间：
1998-02-01
影响因子：
3.2
通讯作者：
Walsh, MC
中科院分区：
文献类型：
--
作者：
Teusink, B;Diderich, JA;Walsh, MC
在表现出高亲和力葡萄糖转运的酿酒酵母细胞中，胞外浓度超过10 mM时，葡萄糖消耗速率仅为零反式转运速率的一半。为了确定这种葡萄糖转运的调节是否可能是细胞内游离葡萄糖的结果，我们开发了一种新的方法来测量代谢葡萄糖的细胞内葡萄糖浓度，该方法将葡萄糖立体异构体与粘着葡萄糖的校正相比较，细胞内葡萄糖浓度为1.5 mM，远远高于大多数早期报道。我们证明，对于最简单的葡萄糖载体模型，该浓度足以减少50%的葡萄糖内流。我们得出结论，细胞内葡萄糖是最有可能被观察到的调节葡萄糖输入和糖酵解的候选物质，我们讨论了细胞内葡萄糖作为主要信号分子在这些细胞中发挥作用的可能性。
In Saccharomyces cerevisiae cells exhibiting high-affinity glucose transport, the glucose consumption rate at extracellular concentrations above 10 mM was only half of the zero trans-influx rate. To determine if this regulation of glucose transport might be a consequence of intracellular free glucose we developed a new method to measure intracellular glucose concentrations in cells metabolizing glucose, which compares glucose stereoisomers to correct for adhering glucose, The intracellular glucose concentration was 1.5 mM, much higher than in most earlier reports. We show that for the simplest model of a glucose carrier, this concentration is sufficient to reduce the glucose influx by 50%. We conclude that intracellular glucose is the most likely candidate for the observed regulation of glucose import and hence glycolysis, We discuss the possibility that intracellular glucose functions as a primary signal molecule in these cells.