AMPK Activation by Dihydrotestosterone Reduces FSH-Stimulated Cell Proliferation in Rat Granulosa Cells by Inhibiting ERK Signaling Pathway
AMPK Activation by Dihydrotestosterone Reduces FSH-Stimulated Cell Proliferation in Rat Granulosa Cells by Inhibiting ERK Signaling Pathway
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DOI：
10.1210/en.2011-1967
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发表时间：
2012-06-01
期刊：
影响因子：
4.8
通讯作者：
Menon, K. M. J.
中科院分区：
文献类型：
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作者：
Kayampilly, Pradeep P.;Menon, K. M. J.
我们之前报道过，5α-二氢睾酮 (DHT) 通过减少细胞周期蛋白 D2 mRNA 表达并阻断 G1/S 期细胞周期进程来抑制 FSH 介导的颗粒细胞增殖。本研究调查了 AMP 激活蛋白激酶 (AMPK) 在 DHT 介导的颗粒细胞增殖抑制中的作用。从 3 天雌二醇引发的未成熟大鼠中收获的颗粒细胞暴露于不同浓度的 DHT（0、45 和 90 ng/ml）24 小时。免疫沉淀 AMPK 的蛋白质印迹分析显示剂量依赖性激活（P
We have previously reported that 5 alpha-dihydrotestosterone (DHT) inhibits FSH-mediated granulosa cell proliferation by reducing cyclin D2 mRNA expression and blocking cell cycle progression at G1/S phase. The present study investigated the role of AMP activated protein kinase (AMPK) in DHT-mediated inhibition of granulosa cell proliferation. Granulosa cells harvested from 3-d estradiol primed immature rats were exposed to different concentrations of DHT (0, 45, and 90 ng/ml) for 24 h. Western blot analysis of immunoprecipitated AMPK showed a dose-dependent activation (P