Vaccinia virus preferentially enters polarized epithelial cells through the basolateral surface.
Vaccinia virus preferentially enters polarized epithelial cells through the basolateral surface.
复制标题
痘苗病毒优先通过基底外侧表面进入极化上皮细胞。
DOI：
10.1128/jvi.65.1.494-498.1991
复制
发表时间：
1991
影响因子：
5.4
通讯作者：
Esteban,M
中科院分区：
文献类型：
--
作者：
Rodriguez,D;Rodriguez,JR;Ojakian,GK;Esteban,M
研究了痘苗病毒在极化上皮细胞中的摄取，以确定进入的位置是限制在根尖膜还是基底外侧膜。用携带荧光素酶报告基因的重组痘苗病毒监测病毒感染。利用MDCK和具有高跨上皮电阻的克隆株MDCK-D11，我们确定痘苗病毒优先通过基底膜进入。文中还讨论了痘苗病毒受体在细胞表面存在极化分布的可能性，这种分布可能与系统性痘病毒感染有关。
The uptake of vaccinia virus in polarized epithelial cells was studied to determine whether the site of entry was confined to either the apical or the basolateral membrane. Virus infection was monitored with a recombinant vaccinia virus carrying the luciferase reporter gene. Using cell lines MDCK and MDCK-D11, a clonal line with high transepithelial electrical resistance, we determined that vaccinia virus preferentially enters through the basolateral membrane. The possibility that there is a polarized cell surface distribution of vaccinia virus receptors which may be involved in systemic poxvirus infections is discussed.