Crystallization of hepatocyte nuclear factor 1beta in complex with DNA.
Crystallization of hepatocyte nuclear factor 1beta in complex with DNA.
复制标题
肝细胞核因子 1β 与 DNA 复合物的结晶。
DOI：
10.1107/s1744309106015168
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Chi,Young-In
中科院分区：
文献类型：
--
作者：
Lu,Peng;Li,Yun;Gorman,Amanda;Chi,Young-In
Hepatocyte nuclear factor 1β (HNF1β) is a member of the POU transcription-factor family and binds the target DNA as a dimer with nanomolar affinity. The HNF1β–DNA complex has been prepared and crystallized by hanging-drop vapor diffusion in 6%(v/v) PEG 300, 5%(w/v) PEG 8000, 8%(v/v) glycerol and 0.1 M Tris pH 8.0. The crystals diffracted to 3.2 Å (93.9% completeness) using a synchrotron-radiation source under cryogenic (100 K) conditions and belong to space group R3, with unit-cell parameters a = b = 172.69, c = 72.43 Å. A molecular-replacement solution has been obtained and structure refinement is in progress. This structure will shed light on the molecular mechanism of promoter recognition by HNF1β and the molecular basis of the disease-causing mutations found in it.
