A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating
A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating
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DOI：
10.1073/pnas.220420397
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发表时间：
2000-10-24
影响因子：
11.1
通讯作者：
Fink, GR
中科院分区：
文献类型：
--
作者：
Guo, B;Styles, CA;Fink, GR
面包酵母的细胞壁含有一个糖基磷脂酰肌醇（GPI）连接的糖蛋白家族，其结构域类似于致病真菌的粘附素。在野生型细胞中，每种蛋白质在不同的发育过程（交配、侵入性生长、细胞-细胞粘附或成丝）中都有独特的功能。将这些发育事件统一起来的是附着力，要么与惰性底物相连，要么与细胞相连。尽管它们的特异性不同，但这些蛋白质中的许多在过度表达时可以相互替代。例如，在单倍体入侵和二倍体成丝的营养生长过程中，Flo11p是必需的，而Fig2p是交配所必需的。当Flo11p和Fig2p过表达时，它们可以参与交配、入侵、成丝和絮凝。Flo11p在交配中提供Fig2p功能的能力取决于其在交配投射的细胞内定位，Fig2p通常在交配细胞的粘附中起作用。我们的数据表明，即使是遥远的家庭成员，如果定位和表达得当，也能保持执行不同功能的能力。
The cell wall of bakers' yeast contains a family of glycosyl-phosphatidylinositol (GPI)-linked glycoproteins of domain structure similar to the adhesins of pathogenic fungi. In wild-type cells each of these proteins has a unique function in different developmental processes (mating, invasive growth, cell-cell adhesion, or filamentation). What unifies these developmental events is adhesion, either to an inert substrate or to a cell. Although they differ in their specificities, many of these proteins can substitute for each other when overexpressed. For example, Flo11p is required during vegetative growth for haploid invasion and diploid filamentation, whereas Fig2p is required for mating. When overexpressed, Flo11p and Fig2p can function in mating, invasion, filamentation, and flocculation. The ability of Flo11p to supply Fig2p function in mating depends on its intracellular localization to the mating projection, where Fig2p normally functions in the adhesion of mating cells. Our data show that even distant family members retain the ability to carry out disparate functions if localized and expressed appropriately.