COMPARATIVE SYSTEMATIC STUDY ON SPIRILLUM-5175, CAMPYLOBACTER AND WOLINELLA SPECIES - DESCRIPTION OF SPIRILLUM-5175 AS SULFUROSPIRILLUM-DELEYIANUM GEN-NOV SPEC NOV
COMPARATIVE SYSTEMATIC STUDY ON SPIRILLUM-5175, CAMPYLOBACTER AND WOLINELLA SPECIES - DESCRIPTION OF SPIRILLUM-5175 AS SULFUROSPIRILLUM-DELEYIANUM GEN-NOV SPEC NOV
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DOI：
10.1007/bf00245247
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发表时间：
1992-09-01
影响因子：
2.8
通讯作者：
PFENNIG, N
中科院分区：
文献类型：
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作者：
SCHUMACHER, W;KRONECK, PMH;PFENNIG, N
采用生理生化试验、HPLC G + C值测定和DNA-DNA杂交等方法，对“螺菌”5175进行了分类归属的研究。将这种兼性硫还原细菌与表型最相似的物种Wolinella succinogenes和Campylobacter acetotorum biovar bubulus的模式菌株进行比较。除了形态学，以下生理特性是共同的：使用元素硫，硝酸盐，亚硝酸盐，天冬氨酸，富马酸盐或苹果酸盐作为电子受体生长与氢或甲酸在缺氧条件下;微需氧生长与2%（v/v）的氧气。Wolinella succinogenes（51.8 mol%）和Campylobacter acutorum biovar bubulus（30.4 mol%）的G + C含量与“Spirillum”5175（40.6 mol%）的G + C含量相差约10 mol%。3株病毒之间没有明显的DNA同源性。这些差异排除了“螺菌”5175与Wolinella或弯曲杆菌属的联系，尽管表型相似。根据我们的结果和其他作者的DNA-rRNA杂交研究，我们为自由生活的弯曲杆菌样细菌“Spirrillum”5175和“Campylobacter spec.”建立了新的硫螺菌属。“DSM 806。菌株“螺菌属”5175被描述为新属和新种Deleyianum Sulfurophyllum的模式菌株。
Physiological tests, redetermination of G + C values with HPLC and DNA-DNA hybridization were used to determine the taxonomic affiliation of "Spirillum" 5175. This facultatively sulfur-reducing bacterium was compared to the type strains of the phenotypically most similar species Wolinella succinogenes and Campylobacter sputorum biovar bubulus. In addition to morphology, the following physiological properties were in common: use of elemental sulfur, nitrate, nitrite, aspartate, fumarate or malate as electron acceptor for growth with hydrogen or formate under anoxic conditions; microaerobic growth with 2% (v/v) oxygen. The G + C content of Wolinella succinogenes (51.8 mol%) and Campylobacter sputorum biovar bubulus (30.4 mol%) differs about 10 mol% from the G + C content of "Spirillum" 5175 (40.6 mol%). No significant DNA homology could be detected between the three strains. These differences excluded affiliation of "Spirillum" 5175 with the genera Wolinella or Campylobacter despite phenotypic similarities. On the basis of our results and DNA-rRNA hybridization studies by other authors, we established the new genus Sulfurospirillum for the free-living Campylobacter-like bacteria "Spirillum" 5175 and "Campylobacter spec." DSM 806. Strain "Spirillum" 5175 is described as the type strain of the new genus and species Sulfurospirillum deleyianum.