Urinary and Fecal Metabonomics Study of the Protective Effect of Chaihu-Shu-Gan-San on Antibiotic-Induced Gut Microbiota Dysbiosis in Rats.
Urinary and Fecal Metabonomics Study of the Protective Effect of Chaihu-Shu-Gan-San on Antibiotic-Induced Gut Microbiota Dysbiosis in Rats.
复制标题
柴胡疏肝散对抗生素引起的大鼠肠道菌群失调保护作用的尿液和粪便代谢组学研究
DOI：
10.1038/srep46551
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发表时间：
2017-04-20
影响因子：
4.6
通讯作者：
Zou ZM
中科院分区：
文献类型：
--
作者：
Yu M;Jia HM;Zhou C;Yang Y;Sun LL;Zou ZM
越来越多的证据表明，肠道菌群失调及其宿主代谢表型改变是人类疾病发展的重要因素。柴胡疏肝散是一种传统的中草药方剂，在治疗各种胃肠道疾病方面有着广泛的应用。本研究旨在探讨在肠道菌群失调的条件下，CSGS是否调节宿主的代谢表型。采用UPLC-Q-TOF/MS技术对抗生素诱导的肠道菌群失调大鼠进行代谢组学研究。偏最小二乘判别分析（PLS-DA）结果表明，CSGS治疗可降低抗生素诱导的代谢表型紊乱。此外，肠道微生物群属与尿液和粪便代谢物之间存在很强的相关性。此外，相关性分析和代谢途径分析（MetPA）确定，三个关键的代谢途径，包括甘氨酸，丝氨酸和苏氨酸代谢，烟酸和烟酰胺代谢，和胆汁酸代谢是最相关的途径参与益生菌诱导的肠道菌群失调。这些发现提供了对CSGS对肠道微生物群失调大鼠的宿主代谢表型的保护作用的全面理解，并进一步作为肠道微生物群靶向疾病管理中药物先导物的新来源。
Accumulating evidence suggests that the gut microbiota dysbiosis and their host metabolic phenotype alteration is an important factor in human disease development. A traditional Chinese herbal formula, Chaihu-Shu-Gan-San (CSGS), has been effectively used in the treatment of various gastrointestinal (GI) disorders. The present study was carried out to investigate whether CSGS modulates the host metabolic phenotype under the condition of gut microbiota dysbiosis. The metabonomics studies of biochemical changes in urine and feces of antibiotic-induced gut microbiota dysbiosis rats after treatment with CSGS were performed using UPLC-Q-TOF/MS. Partial least squares-discriminate analysis (PLS-DA) indicated that the CSGS treatment reduced the metabolic phenotype perturbation induced by antibiotic. In addition, there was a strong correlation between gut microbiota genera and urinary and fecal metabolites. Moreover, the correlation analysis and the metabolic pathway analysis (MetPA) identified that three key metabolic pathways including glycine, serine and threonine metabolism, nicotinate and nicotinamide metabolism, and bile acid metabolism were the most relevant pathways involved in antibiotic-induced gut microbiota dysbiosis. These findings provided a comprehensive understanding of the protective effects of CSGS on the host metabolic phenotype of the gut microbiota dysbiosis rats, and further as a new source for drug leads in gut microbiota-targeted disease management.