The lamin Dm0 allele Ari3 acts as an enhancer of position effect variegation of the wm4 allele in Drosophila
The lamin Dm0 allele Ari3 acts as an enhancer of position effect variegation of the wm4 allele in Drosophila
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DOI：
10.1007/s10709-006-0012-7
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发表时间：
2007-03-01
期刊：
影响因子：
1.5
通讯作者：
Johansen, Kristen M.
中科院分区：
文献类型：
--
作者：
Bao, Xiaomin;Girton, Jack;Johansen, Kristen M.
层粘连蛋白和层粘连蛋白结合蛋白与外周异染色质的关联提示层粘连蛋白可能通过参与染色质结构的表观遗传调控来影响基因表达。为了验证这一假设，我们研究了最近产生的部分功能缺失的层状蛋白Dm(0)等位基因Ari3对果蝇眼睛中w （m4）等位基因PEV的影响。Lam （Ari3）等位基因的特征是cooh末端结构域的截断，并且缺乏将层状蛋白定位于核膜的CaaX盒。我们发现Lam （Ari3）等位基因强烈地增加了w （m4）表达的沉默，从而作为PEV的增强子。这些结果表明，层粘连蛋白可能参与调控w （m4）位点的基因沉默和异色扩散，并为层粘连蛋白可能参与高阶染色质组织的调控提供了证据。
The association of lamin and lamin binding proteins with peripheral heterochromatin suggests the possibility that lamins may influence gene expression by participating in the epigenetic regulation of chromatin stucture. To test this hypothesis we have examined the effect of a recently generated partial loss-of-function lamin Dm(0) allele Ari3 on PEV of the w (m4) allele in the Drosophila eye. The Lam (Ari3) allele is characterized by a truncation of the COOH-terminal domain and lacks the CaaX box that localizes lamin to the inner nuclear membrane. We show that the Lam (Ari3) allele strongly increased silencing of w (m4) expression, thus acting as an enhancer of PEV. These results indicate that lamins may be involved in regulating gene silencing and heterochromatic spreading at the w (m4) locus and provide evidence that lamins may contribute to the regulation of higher-order chromatin organization.