Increasing CO2 changes community composition of pico- and nano-sized protists and prokaryotes at a coastal Antarctic site
Increasing CO2 changes community composition of pico- and nano-sized protists and prokaryotes at a coastal Antarctic site
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二氧化碳浓度的增加改变了南极沿海地区皮微型和纳米尺寸原生生物和原核生物的群落组成
DOI：
10.3354/meps11803
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发表时间：
2016
影响因子：
2.5
通讯作者：
L. Maher
中科院分区：
文献类型：
--
作者：
P. Thomson;A. Davidson;L. Maher
海洋酸化是一种全球公认的现象，但人们对其对南极海洋微生物的影响知之甚少。在这里，我们报告的皮科和nanophytoplankton，异养nanoflagellates（HNF）和原核生物（微藻和细菌）在3 minicosm实验在2008/2009年夏天在南极洲戴维斯站，CO2浓度升高的社区响应。在650个小型海洋生物（n = 6）中培养12 d，培养时的CO2浓度范围从工业化前的水平到2100年及以后的预测水平。通过流式细胞术测定皮科和纳米级原生生物和原核生物的丰度，使用叶绿素自发荧光来区分浮游植物，SYBR-绿色染色原核生物，LysoTracker绿色染色来区分HNF。虽然对纳米浮游植物丰度的影响尚无定论，但我们的研究结果表明，增加二氧化碳可以改变南极沿海沃茨水域微生物群落的组成。我们的3个实验一致表明，较低浓度的HNF和高丰度的picophytoplankton和原核生物在处理暴露于高浓度的CO2。虽然机制仍有待证实，我们的研究表明，CO2可能会减少死亡的微微浮游生物的HNF放牧。我们的研究结果表明，南极微生物群落组成的变化可能发生在750至1118 ppm的二氧化碳浓度范围内，可能会影响南极食物网通过减少食物供应。
Ocean acidification is a globally recognised phenomenon, but little is known of its impacts on Antarctic marine microbes. Here we report on the community response of pico- and nanophytoplankton, heterotrophic nanoflagellates (HNF) and prokaryotes ( Archaea and Bacteria ) to elevated CO 2 during 3 minicosm experiments over the 2008/2009 summer at Davis Station, Antarctica. Coastal seawater was incubated in 650 l minicosms ( n = 6) for ≤ 12 d at CO 2 concentrations ranging from preindustrial levels to those predicted for 2100 and beyond. The abundance of pico- and nano-sized protists and prokaryotes were determined by flow cytometry, using chlorophyll autofluorescence to discriminate the phytoplankton, SYBR-Green to stain the prokaryotes and LysoTracker Green stain to discriminate the HNF. While the effects on nanophytoplankton abundance were inconclusive, our results show that increasing CO 2 can alter the composition of the microbial community in Antarctic coastal waters. Our 3 experiments consistently showed lower concentrations of HNF and higher abundances of picophytoplankton and prokaryotes in treatments exposed to elevated CO 2 . While the mechanism remains to be confirmed, our study suggests that CO 2 may reduce the mortality of picoplankton by HNF grazing. Our results indicate that changes in the composition of Antarctic microbial communities may occur within the concentration range of 750 to 1118 ppm CO 2 , potentially impacting the Antarctic food web through reduced food availability.
影响因子：
4.5
作者：
S. Trimborn;Tina Brenneis;E. Sweet;B. Rost
通讯作者：
S. Trimborn;Tina Brenneis;E. Sweet;B. Rost
影响因子：
3.7
作者：
Endres S;Galgani L;Riebesell U;Schulz KG;Engel A
通讯作者：
Engel A
影响因子：
18.3
作者：
Lohbeck, Kai T.;Riebesell, Ulf;Reusch, Thorsten B. H.
通讯作者：
Reusch, Thorsten B. H.