Endothelial cell dynamics during anastomosis in vitro.
Endothelial cell dynamics during anastomosis in vitro.
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体外吻合过程中的内皮细胞动力学。
DOI：
10.1039/c5ib00052a
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发表时间：
2015-04
期刊：
影响因子：
--
通讯作者：
Stroock AD
中科院分区：
文献类型：
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作者：
Diaz-Santana A;Shan M;Stroock AD
血管吻合-来自血管系统的两个不同分支的血管的融合-代表了在健康和病理条件下体内血管生长的关键步骤，并且为体外血管化组织的工程化提供了重要目标。最近在动物模型中的工作推进了我们对血管吻合中分子和细胞参与者的理解，但问题仍然与体外细胞动力学和该过程的控制有关。在这项研究中，我们利用三维（3-D）培养平台，检查血管吻合术期间和之后的内皮细胞（EC）的动态，允许血管生成和血管发生并行进行。我们发现，吻合发生在血管生成形成的芽从内皮和管形成的大部分的3-D矩阵中。这种融合导致高度连接的血管以取决于细胞密度和身份的方式从基质表面跨越到本体中。此外，我们观察并分析了不同来源的内皮细胞（表面与体积）在形成的血管结构内的混合;我们提供了细胞沿沿着预先存在的血管段迁移作为这种混合过程的一部分的证据。我们的结论是吻合可以发生在血管新生和血管发生的血管之间，这个过程可能在伤口愈合等方面发挥重要作用。
Vascular anastomosis –the fusion of vessels from two distinct branches of the vascular system – represents a critical step in vascular growth under both healthy and pathological conditions, in vivo, and presents an important target for engineering of vascularized tissues, in vitro. Recent works in animal models have advanced our understanding of the molecular and cellular players in vascular anastomosis, but questions remain related to cellular dynamics and control of this process, in vitro. In this study, we exploited a three-dimensional (3-D) culture platform to examine the dynamics of endothelial cell (EC) during and after vascular anastomosis by allowing angiogenesis and vasculogenesis to proceed in parallel. We show that anastomosis occurs between sprouts formed by angiogenesis from an endothelium and tubes formed by vasculogenesis in the bulk of a 3-D matrix. This fusion leads to highly connected vessels that span from the surface of the matrix into the bulk in a manner that depends on cell density and identity. Further, we observe and analyze intermixing of endothelial cells of distinct origin (surface versus bulk) within the vessels structures that are formed; we provide evidence that the cells migrate along pre-existing vessels segments as part of this intermixing process. We conclude that anastomosis can occur between vessels emerging by angiogenesis and vasculogenesis and that this process may play an important role in contexts such as wound healing.