Daiokanzoto (Da-Huang-Gan-Cao-Tang) is an effective laxative in gut microbiota associated with constipation
Daiokanzoto (Da-Huang-Gan-Cao-Tang) is an effective laxative in gut microbiota associated with constipation
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DOI：
10.1038/s41598-019-40278-2
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发表时间：
2019-03-07
影响因子：
4.6
通讯作者：
Okamura, Nobuyuki
中科院分区：
文献类型：
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作者：
Takayama, Kento;Takahara, Chiho;Okamura, Nobuyuki
番泻苷A（SA）和大黄甘草汤（Daiokanzoto，DKT）的泻下作用受个体差异的影响。在这项研究中，我们操纵小鼠的肠道微生物群，通过给它们喂食高碳水化合物，高脂肪或高纤维饮食来建立泻药反应者和无反应者。为了评估泻药和肠道微生物群之间的关系，我们在给予泻药之前和之后监测了肠道微生物群。将每种饮食的20只小鼠分为4组，每组5只小鼠，以评价4种泻药制剂DKT、SA、SA+大黄酸8-O-β-D-吡喃葡萄糖苷（SA + RG）和SA+甘草苷（SA + LQ）的通便活性。通过16 S rRNA基因扩增子的下一代测序监测肠道微生物群的变化。在高碳水化合物和高脂肪饮食喂养的小鼠中，DKT比单独的SA发挥出显着更高的通便活性，而RG对这种活性做出了贡献。DKT和SA + RG给药增加了肠道微生物群的肠杆菌科含量，这与增加的通便活性相关。相反，DKT活性显着抑制高纤维饮食。因此，饮食诱导的肠道微生物群差异决定了DKT的作用，考虑到东方药物是针对特定的功能状态或“模式”配制的，这是有趣的。这些结果表明，蒽类泻药的泻下活性易受饮食诱导的肠道微生物群改变的影响。
Interindividual differences affect the purgative activities of sennoside A (SA) and Daiokanzoto (Da-Huang-Gan-Cao-Tang, DKT). In this study, we manipulated gut microbiota in mice to establish laxative responders and non-responders by feeding them a high-carbohydrate, a high-fat or a high-fibre diet. To assess the relationship between laxatives and gut microbiota, we monitored the gut microbiota before and after administering laxatives. Twenty mice per diet were divided into four groups of five mice to evaluate purgative activities of four laxative preparations, DKT, SA, SA plus rhein 8-O-beta-D-glucopyranoside (SA + RG), and SA plus liquiritin (SA + LQ). Gut microbiota changes were monitored by next-generation sequencing of 16 S rRNA gene amplicons. In high-carbohydrate and high-fat diet-fed mice, DKT exerted a significantly higher purgative activity than SA alone, and RG contributed to this activity. DKT and SA + RG administration increased the Enterobacteriaceae content of gut microbiota, which was associated with an increased purgative activity. In contrast, DKT activity was significantly suppressed by high-fibre diet. Hence, diet-induced differences in gut microbiota determined the effect of DKT, which is interesting, considering that Oriental medicines are formulated for a specific functional state or "pattern". These results demonstrated that the purgative activity of anthranoid laxatives is susceptible to diet-induced alterations in gut microbiota.