7-hydroxy-3-(4-hydroxybenzyl)chroman and broussonin b: neurotrophic compounds, isolated from Anemarrhena asphodeloides BUNGE, function as proteasome inhibitors.
7-hydroxy-3-(4-hydroxybenzyl)chroman and broussonin b: neurotrophic compounds, isolated from Anemarrhena asphodeloides BUNGE, function as proteasome inhibitors.
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DOI：
10.1248/bpb.28.1798
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发表时间：
2005-09
影响因子：
2
通讯作者：
S. Tsukamoto;Takeshi Wakana;Keiichiro Koimaru;Takushi Yoshida;Mako Sato;T. Ohta
中科院分区：
文献类型：
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作者：
S. Tsukamoto;Takeshi Wakana;Keiichiro Koimaru;Takushi Yoshida;Mako Sato;T. Ohta
知母提取物对大鼠嗜铬细胞瘤（PC-12）细胞具有神经营养活性。生物测定引导纯化得到四个化合物，2，6，4 '-三羟基-4-甲氧基二苯甲酮（1）、7-羟基-3-（4-羟基苄基）苯并二氢吡喃（2）、broussonin B（3）和顺式-扁柏树脂酚（4）。化合物1-3在50 μ g/ml的浓度下诱导PC-12细胞中的神经突生长，而4活性较低。此外，化合物2-4对蛋白酶体的胰凝乳蛋白酶样活性显示出中等的抑制活性。
The extract of Anemarrhenae Rhizoma (rhizomes of Anemarrhena asphodeloides BUNGE) showed neurotrophic activity toward rat pheochromocytoma (PC-12) cells. Bioassay-guided purification afforded four compounds, 2,6,4'-trihydroxy-4-methoxybenzophenone (1), 7-hydroxy-3-(4-hydroxybenzyl)chroman (2), broussonin B (3), and cis-hinokiresinol (4). Compounds 1-3 induced neurite outgrowth in PC-12 cells at concentration of 50 microg/ml, while 4 was less active. In addition, compounds 2-4 showed moderate inhibitory activities against a chymotrypsin-like activity of the proteasome.