Responses of olfactory receptor cells of spiny lobsters to binary mixtures. I. Intensity mixture interactions.
Responses of olfactory receptor cells of spiny lobsters to binary mixtures. I. Intensity mixture interactions.
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大龙虾嗅觉受体细胞对二元混合物的反应。
DOI：
10.1152/jn.1991.66.1.112
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发表时间：
1991
影响因子：
2.5
通讯作者：
P. C. Daniel
中科院分区：
文献类型：
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作者：
C. Derby;M. Girardot;P. C. Daniel
1.利用多刺龙虾的外周嗅觉系统研究了化学混合物的神经编码。混合物相互作用的发生（即，其中观察到的对混合物的响应显著偏离预期响应），并检测了这种混合物相互作用对细胞群体编码气味强度的影响。2.测量了触角中98个初级嗅觉受体细胞对7种化合物[5 '-一磷酸腺苷（AMP）、甜菜碱（Bet）、L-半胱氨酸（Cys）、L-谷氨酸（Glu）、氯化铵（NH 4）、DL-琥珀酸（Suc）和牛磺酸（Tau）]及其二元混合物的反应的细胞外记录。为了确定混合物的相互作用，将观察到的对二元混合物浓度范围的响应与基于三种数学模型的预测响应进行比较：单受体模型，其假设混合物的两种化合物结合到相同的受体位点;多受体模型，其假设两种化合物结合到两个独立的受体位点;和一个混合组成的受体模型，其中包括我们目前的知识状态的转导过程中的嗅觉受体细胞的多刺龙虾。3.单个细胞中的混合物相互作用很常见：在25%的可能情况下观察到统计学显著的混合物相互作用（图5）。压制，比强化要常见的多。4.混合物相互作用对细胞群体的绝对反应幅度有显著影响，这可以用作该系统中刺激强度的神经代码。这些效应被称为强度混合相互作用（图1和图2）。6-11）。强度混合物相互作用发生在大约50%的二元混合物中，并且几乎完全是抑制的（图1和图2）。12和13）。混合物相互作用的强度与浓度无关。5.结果表明，在个别嗅觉细胞中的混合物相互作用可以导致在神经元群体中的强度混合物相互作用，使得相对于基于对混合物的个别组分的响应所预期的，对二元混合物的敏感性降低。
1. Neural coding of chemical mixtures was studied with the use of the peripheral olfactory system of the spiny lobster. The occurrence of mixture interactions (i.e., where the observed response to a mixture deviates significantly from the expected response) in individual cells and the effect of such mixture interactions on the coding of odorant intensity by populations of cells were examined. 2. Extracellular recordings of spiking activity of 98 primary olfactory receptor cells in the antennules were measured in response to seven compounds [adenosine-5'-monophosphate (AMP), betaine (Bet), L-cysteine (Cys), L-glutamate (Glu), ammonium chloride (NH4), DL-succinate (Suc), and taurine (Tau)] and their binary mixtures. To identify mixture interactions, observed responses to a range of concentrations of a binary mixture were compared with the predicted responses based on three mathematical models: a single receptor model, which assumes that the two compounds of a mixture bind to the same receptor site; a multiple receptor model, which assumes that the two compounds bind to two independent receptor sites; and a mixed composition receptor model, which incorporates our current state of knowledge of transduction processes in olfactory receptor cells of spiny lobsters. 3. Mixture interactions in individual cells were common: statistically significant mixture interactions were observed in 25% of the possible cases (Fig. 5). Suppression was much more common than enhancement. 4. Mixture interactions had significant effects on the absolute response magnitudes for a population of cells, which could be used as the neural code for stimulus intensity in this system. These effects are called intensity mixture interactions (Figs. 6-11). Intensity mixture interactions occurred for approximately 50% of the binary mixtures and were almost exclusively suppression (Figs. 12 and 13). The intensity mixture interactions were concentration independent. 5. The results suggest that mixture interactions in individual olfactory cells can result in intensity mixture interactions in the neuronal population such that there is a decrease in sensitivity to binary mixtures relative to what is expected based on the responses to individual components of the mixtures.