HTLV‐I DNA Sequences in CNS Tissue of a Patient with Tropical Spastic Paraparesis and HTLV‐I‐Associated Myelopathy
HTLV‐I DNA Sequences in CNS Tissue of a Patient with Tropical Spastic Paraparesis and HTLV‐I‐Associated Myelopathy
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热带痉挛性截瘫和 HTLV-I 相关脊髓病患者中枢神经系统组织中的 HTLV-I DNA 序列
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发表时间：
1992
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影响因子：
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通讯作者：
C. Gibbs
中科院分区：
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作者：
R. Iannone;M. Sherman;P. Rodgers;M. Beilke;C. Mora;Rajnikant M. Amin;Susan R. Tinsley;L. Papsidero;B. Poiesz;C. Gibbs
人类 T 细胞淋巴病毒 I 型 (HTLV-1) 是成人 T 细胞淋巴瘤/白血病 (ATLL) 和热带痉挛性截瘫/HTLV-I 相关脊髓病 (TSP/HAM) 的病原体。我们研究了一名 HTLV-I 血清反应阳性的白人，他于 1977 年被诊断患有 ATLL，10 年后，即他去世前 6 个月，诊断为 TSP/HAM。对脑、脊髓和内脏组织切片进行组织学、免疫组织化学、原位杂交和聚合酶链式反应 (PCR) 检查，对内脏组织中 HTLV-I 聚合酶 (pol) 基因区域的 PCR 扩增表明，肝脏石蜡包埋切片以及从肾脏和脾脏冷冻切片提取的 DNA 中存在前病毒 HTLV-I DNA，但未能证明病毒存在肺和淋巴结石蜡切片中的序列。对中枢神经系统组织的 PCR 分析显示了大脑区域中的病毒序列，包括小脑和大脑皮层的冷冻样本以及胸脊髓的石蜡切片，但未能在腰脊髓区域的切片中检测到前病毒 DNA。这些结果绘制了 3 个月的 TSP/HAM 患者 CNS 中 HTLV-I DNA 序列的分布情况。
Human T cell lymphotrophic virus type I (HTLV-1) is the etiologic agent of adult T cell lymphoma/leukemia (ATLL) and tropical spastic paraparesis/HTLV-I-associated myelopathy (TSP/HAM). We studied an HTLV-I-seropositive, white man diagnosed in 1977 with ATLL and 10 years later, 6 months prior to his death, with TSP/HAM. Sections of brain, spinal cord, and visceral tissues were examined histologically, immunohistochemically, by in situ hybridization, and by the polymerase chain reaction (PCR), PCR amplification of a region of the polymerase (pol) gene of HTLV-I from visceral tissue demonstrated the presence of proviral HTLV-I DNA in paraffin-embedded sections from the liver and in DNA extracted from frozen sections of kidney and spleen, but failed to demonstrate viral sequences in paraffin sections of the lung and a lymph node. PCR analysis of CNS tissue demonstrated viral sequences in regions of the brain including frozen samples from cerebellum and cerebral cortex and paraffin sections of the thoracic spinal cord, but failed to detect proviral DNA in sections from a region in the lumbar cord. These results map the distribution of HTLV-I DNA sequences in the CNS of a patient with TSP/HAM for 3 months.