Nuclear DNA, chloroplast DNA, and ploidy analysis clarified biological complexity of the Vandenboschia radicans complex (Hymenophyllaceae) in Japan and adjacent areas
Nuclear DNA, chloroplast DNA, and ploidy analysis clarified biological complexity of the Vandenboschia radicans complex (Hymenophyllaceae) in Japan and adjacent areas
复制标题
DOI：
10.3732/ajb.92.9.1535
复制
发表时间：
2005-09-01
影响因子：
3
通讯作者：
Ito, M
中科院分区：
文献类型：
--
作者：
Ebihara, A;Ishikawa, H;Ito, M
由定义不清的“物种”组成的物种复合体在蕨类植物中广为人知，它们与网状进化的参与是预期的。然而，用DNA标记来研究网状历史的方法尚未被普遍采用。通过倍性水平、cpDNA标记（rbcL）和核DNA标记（GapC）分析，我们成功地阐明了东亚岛屿上的范登博氏菌复合体的生物学地位。从日本和台湾的174个地点收集的266个个体的结果表明，至少有3个亲本二倍体种来自于V. radicans复合体和Vandenboschia liukiuensis（以前被认为与该复合体不同）。三倍体是最常见的细胞类型，但它们没有证据表明无精生殖，而所有非杂交二倍体是罕见的，分布非常有限。对这一现象的可能解释将简要地讨论，包括遗存分布的可能性和偶尔的无精生殖。
Species complexes consisting of ill-defined "species" are widely known among ferns, and their involvement with reticulate evolution is expected. Nevertheless approaches to reticulation history with DNA markers are not yet commonly adopted. We have successfully elucidated the biological status of the Vandenboschia radicans complex in East Asian islands by combining analyses of ploidy level, a cpDNA marker (rbcL), and a nuclear DNA marker (GapC). The results based on 266 individuals collected from 174 localities throughout Japan and Taiwan suggest that complicated hybridizations have occurred involving at least three parental diploid species from within the V. radicans complex and Vandenboschia liukiuensis, which was formerly considered to be distinct from this complex. Triploids are the most common cytotype, but they show no evidence of apogamous reproduction, while all nonhybrid diploids are rare and have very limited distribution. Possible accounts of this phenomenon will be briefly discussed including the possibility of relict distribution and occasional apogamous reproduction.