CHANGES IN SPERM SURFACE-MEMBRANE AND LUMINAL PROTEIN FLUID CONTENT DURING EPIDIDYMAL TRANSIT IN THE BOAR
CHANGES IN SPERM SURFACE-MEMBRANE AND LUMINAL PROTEIN FLUID CONTENT DURING EPIDIDYMAL TRANSIT IN THE BOAR
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DOI：
10.1095/biolreprod40.3.635
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发表时间：
1989-03-01
影响因子：
3.6
通讯作者：
PAQUIGNON, M
中科院分区：
文献类型：
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作者：
DACHEUX, JL;DACHEUX, F;PAQUIGNON, M
对猪精子在附睾转运过程中的表面膜蛋白和配子周围液蛋白进行了分析。本研究表明，在附睾运输过程中，精子膜和附睾液的蛋白质组成发生了连续的戏剧性变化。猪精子表面的成熟过程是精子膜大分子组成和取向的复杂连续演化过程。附睾成熟导致大多数睾丸表面化合物逐渐消失，这些化合物被猪精子表面膜上的永久或短暂的低分子量多肽更新或掩盖。在精子周围的液体中，管腔蛋白的组成在整个附睾运输过程中发生了变化，并发现了几种新的化合物。很少有蛋白质与血浆或精子表面化合物相关。
The surface membrane protein of boar sperm and the proteins in the fluid surrounding the gametes were analyzed during epididymal transit. The present study demonstrated that sequential dramatic changes occur in protein composition of the sperm membrane and epididymal fluid during epididymal transit. The maturation process of the boar sperm surface was characterized by a complex sequential evolution of the composition and orientation of macromolecules in the sperm membrane. Epididymal maturation resulted in the progressive disappearance of most of the surface testicular compounds, which were either renewed or masked by bew permanent or transient low molecular weight polypeptides on the boar sperm surface membrane. In the fluid surrounding the spermatozoa, composition of the luminal proteins was altered throughout the epididymal transit and several new compounds were characterized. Very few proteins were correlated either with blood plasma or sperm surface compounds.