Daily exercise normalizes the number of diaphorase (NOS) positive neurons in the hypothalamus of hypertensive rats
Daily exercise normalizes the number of diaphorase (NOS) positive neurons in the hypothalamus of hypertensive rats
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DOI：
10.1016/s0006-8993(02)03400-5
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发表时间：
2002-11-15
期刊：
影响因子：
2.9
通讯作者：
Patel, KP
中科院分区：
文献类型：
--
作者：
DiCarlo, SE;Zheng, H;Patel, KP
众所周知，下丘脑室旁核（PVN）内的一氧化氮（NO）通过抑制性GABA能机制介导交感神经抑制。此外，在自发性高血压大鼠（SHR）中，抑制性GABA能机制受损。这些数据表明，一氧化氮系统，在PVN，也可能是受损的SHR。此外，先前的研究已经证明，日常锻炼减弱了SHR中心动过速、高血压和血压相关心血管疾病危险因素的发展。这些数据表明，日常锻炼增强抑制GABA能和/或NO系统。因此，本研究旨在验证以下假设：SHR中的高血压与PVN内NADPH-黄递酶（神经元NOS活性的常用标记物）阳性神经元的数量减少相关，并且每日运动增加NOS阳性神经元的数量。采用标准组织化学方法，在PVN、视上核、正中视前区、外侧下丘脑、孤束核和延髓头端腹外侧区测定NOS阳性神经元。结果表明，SHR在PVN中的NOS阳性神经元明显少于其遗传对照，Wistar-Kyoto（WKY）大鼠（110+/-11 vs 139+/-17）。此外，日常运动增加了SHR中NOS阳性神经元的数量，达到WKY大鼠的水平。这些数据表明，在SHR中，高血压与PVN内NOS阳性神经元的数量减少相关，并且每日运动增加PVN内NOS阳性神经元的数量。(C)2002 Elsevier Science B. V.保留所有权利。
It is well known that nitric oxide (NO), within the paraventricular nucleus (PVN) of the hypothalamus, mediates sympatho-inhibition via an inhibitory GABA-ergic mechanism. Furthermore, the inhibitory GABA-ergic mechanism is impaired in the spontaneously hypertensive rat (SHR). These data suggest that the NO system, within the PVN, may also be impaired in the SHR. In addition, previous studies have documented that daily exercise attenuates the development of tachycardia, hypertension and blood pressure related cardiovascular disease risk factors in SHR. These data suggest that daily exercise enhances the inhibitory GABA-ergic and/or NO systems. Therefore, this study was designed to test the hypothesis that hypertension, in the SHR, is associated with a lower number of NADPH-diaphorase (a commonly used marker for neuronal NOS activity) positive neurons within the PVN and that daily exercise increases the number of NOS positive neurons. Using a standard histochemical protocol, NOS positive neurons were measured in the PVN, supraoptic nucleus, median preoptic area, lateral hypothalamus, nucleus of the tractus solitarius and rostral ventrolateral medulla. Results document that SHR have significantly fewer NOS-positive neurons in the PVN than their genetic control, the Wistar-Kyoto (WKY) rats (110+/-11 versus 139+/-17). Furthermore, daily exercise increased the number of NOS positive neurons in the SHR to levels seen in the WKY rats. These data demonstrate that hypertension, in the SHR, is associated with a lower number of NOS positive neurons within the PVN and that daily exercise increases the number of NOS positive neurons within the PVN. (C) 2002 Elsevier Science B.V. All rights reserved.