Decreased endothelial size and connexin expression in rat caudal arteries during hypertension
Decreased endothelial size and connexin expression in rat caudal arteries during hypertension
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DOI：
10.1097/00004872-200202000-00014
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发表时间：
2002-02-01
影响因子：
4.9
通讯作者：
Hill, CE
中科院分区：
文献类型：
--
作者：
Rummery, NM;McKenzie, KUS;Hill, CE
目的高血压伴血管内皮功能障碍。本研究观察了自发性高血压雄性大鼠(SHR)在高血压形成过程中尾动脉内皮细胞形态和连接蛋白的表达。方法9周龄自发性高血压雄性大鼠(SHR)较正常血压Wistar-京都(WKY)大鼠的收缩压显著升高，11-12周龄时达到最大。用免疫组织化学方法检测高血压前期(3周龄)和高血压(12周龄)大鼠内皮细胞大小及连接蛋白(CXS)37、40和43的表达。结果12周龄SHR大鼠血管内皮细胞的大小和3种CXS的表达均显著低于WKY大鼠。3周时细胞大小和CXS37、43的表达无明显差异，但SHR的Cx40表达明显低于WKY大鼠。在WKY大鼠3-12周，内皮细胞大小没有变化，CXS 37、40和43的表达也没有变化。SHR在同一发育时期内皮细胞大小和CxS表达均显著减少，Cx40斑块密度显著降低。结论SHR高血压时内皮细胞大小和Cx40表达显著减少，这可能是高血压时内皮细胞功能紊乱的原因之一。J Hyperten 20：247-253(C)2002 Lippincott Williams Wilkins.
Objectives Hypertension is accompanied by endothelial dysfunction. The present study has investigated endothelial cell morphology and connexin expression in the caudal artery of the rat during the development of hypertension.Methods A significant increase in systolic blood pressure was detected from 9 weeks of age in spontaneously hypertensive male rats (SHR) compared to normotensive Wistar-Kyoto (WKY) rats, reaching a maximum by 11-12 weeks of age. lmmunohistochemistry was used to quantify cell size and expression of connexins (Cxs) 37, 40 and 43 in the endothelium of prehypertensive (3-week-old) and hypertensive (12-week-old) rats.Results At 12 weeks, the size of endothelial cells and the expression of all three Cxs per endothelial cell were significantly less in SHR than WKY rats. At 3 weeks, there was no significant difference in cell size nor in the expression of Cxs 37 or 43; however, expression of Cx40 was significantly lower in SHR than in WKY rats. Between 3 and 12 weeks in WKY rats, there was no change in endothelial cell size, nor in the expression of Cxs 37, 40 and 43. In SHR, both cell size and Cxs expression per endothelial cell were significantly decreased during the same developmental period, with a significant decrease in the density of Cx40 plaques.Conclusion The development of hypertension in the SHR is accompanied by significant decreases in endothelial cell size and expression of Cx40, which may contribute to the endothelial dysfunction present in hypertension. J Hypertens 20:247-253 (C) 2002 Lippincott Williams Wilkins.